晚期混合裂变产物内照射诱发体细胞HPRT基因位点突变率与剂量率关系的研究  

STUDY ON THE RELATIONSHIP BETWEEN DOSE-RATES AND SOMATIC CELL MUTATION AT HPRT LOCUS INDUCED BY LATE FISSION PRODUCTS

作　　者：徐永忠[1] 劳勤华[1] 赵经涌[1] 

机构地区：[1]苏州医学院放射医学系,苏州215007

出　　处：《工业卫生与职业病》1998年第2期65-68,共4页Industrial Health and Occupational Diseases

摘　　要：运用多核细胞法及胞质分裂阻断微核（CBMN）法对5组Wistar雄性大鼠外周血淋巴细胞HPRT基因位点突变频率及微核进行检测。实验组注射不同放射性比活度的晚期混合裂变产物，在达到累积剂量近似相等（约4.66cSv）时心脏穿刺取血。结果显示，在总累积剂量近似相等条件下，HPRT位点突变频率、微核细胞率、微核率均随剂量率的增加而增加，4个剂量率点间的HPRT位点突变频率、微核细胞率和微核率总体上均有显著差异（P＜0．01）。HPRT基因位点突变频率、微校率与剂量率之间的关系可分别用函数Y=a＋blnX表示。HPRT位点突变频率与微核率间存在线性相关。With the technique of multinucleus cell and the method of cytokinesis-b1ock micronticleus(CBMN),the relationships between mutation at HPRT loci,micronucleus and dose-rates were ana-lyzed. The results were as follows:When the accumulative doses of radiation of rats tended to beequal (4. 66cSv),mutant frequencies at HPRT loci,micronucleus cell frequencies,micronuc1eus fre-quencies increased with the rise of dose-rate. Both the relationships between mutant frequencies atHPRT loci,micronucleus frequencies and accumulative doses could be described by following re-gression equation:Y=a+b lnX. A good linear relationship between HPRT mutant frequencies andmicronucleus frequencies was observed.

关 键 词：混合裂变产物 HPRT基因 微核 剂量率效应 

分 类 号：R144.1[医药卫生—公共卫生与预防医学] Q345.2[生物学—遗传学]