广州地区散发性戊型肝炎病毒基因片段序列分析  

Nucleic acid sequence analysis of the sporadic hepatitis E virus strains in Guangzhou

作　　者：魏绍静[1,2] PeterWalsh 唐漾波[1,2] 董惠卿 蔡晓莉[1,2] 

机构地区：[1]广州市传染病医院肝炎研究所 [2]香港理工大学医疗系

出　　处：《中华微生物学和免疫学杂志》1998年第2期92-95,共4页Chinese Journal of Microbiology and Immunology

基　　金：香港1995年CHROUCH基金;广州市科技基金

摘　　要：目的对广州地区散发性戊型肝炎病毒（ＨＥＶ）作基因片段序列分析。方法用逆转录套式聚合酶链反应（ＲＴ－ｎｅｓｔｅｄＰＣＲ）检测了８例广州地区散发性戊肝患者粪便和血清中的ＨＥＶＲＮＡ，其中３例粪便阳性。对阳性ＰＣＲ产物进行基因克隆、核酸序列分析。结果广州地区Ｇ１、Ｇ２、Ｇ３株与缅甸株（Ｂ）、巴基斯坦株（Ｐ）、墨西哥株（Ｍ）、中国新疆株（Ｃｈ１．１）和广州血清株（Ｇ－９）的核苷酸和氨基酸的同源性均值分别为８０．６７％和８８．６０％（Ｂ）、８１．２５％和８９．２０％（Ｐ）、７７．４５％和８４．８１％（Ｍ）、８１．２５％和８９．２０％（Ｃ）、９７．８５％和９６．２０％（Ｇ）。结论广州ＨＥＶ株有一定程度的变异。There were sporadic cases of hepatitis E in Guangzhou.In order to analyze the nucleotide sequence of sporadic HEV strain in Guangzhou, RTnested PCR was used to determine HEV RNA in stool and serum samples of 8 patients with hepatitis E.Three stool samples were positive for HEV RNA. The hemology of nucleic acid and amino acid sequences of 3 HEV strains in Guangzhou compared with the Burmese,Pakistani,Mexican,Chinese Ch1.1 and G9 strains were 80.67% and 88.60%(B),81.25% and 89.20%(P),77.45% and 84.81%(M),81.25% and 89.20%(C),97.85% and 96.20%(G),respectively.The results suggest that HEV strains in Guangzhou have genomutation in some extent.

关 键 词：戊型肝炎病毒 序列分析 基因 聚合酶链反应 

分 类 号：R373.21[医药卫生—病原生物学]