用合成寡肽的酶免疫法检测丙型肝炎病毒血清型  

The ELISA method with synthetic peptides to detect the serotypes of hepatitis C virus

作　　者：陈志[1,2] 王宇[1,2] 陈红松[1,2] 颜学兵 陶其敏 

机构地区：[1]北京医科大学人民医院肝病研究所 [2]徐州医学院第一附属医院

出　　处：《中华微生物学和免疫学杂志》1998年第2期162-164,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨适合我国临床广泛推广应用的丙型肝炎分型方法。方法采用丙型肝炎病毒核心区及ＮＳ４区合成寡肽建立酶联免疫检测方法，对血清中丙型肝炎病毒抗体进行分型。用此方法对某地个体供血者及慢性肝病患者的血清标本进行测定。首先使用美国ＡｂｂｏｔＩＭｘ试剂测定抗－ＨＣＶ，阳性者再测定其血清型。为验证血清分型与基因型的关系，对ＨＣＶＲＮＡ阳性的标本用型特异性引物的分型ＰＣＲ方法测定其基因型。结果７０例已往单采血浆供血者中ＨＣＶＲＮＡ阳性为３０％，抗－ＨＣＶ阳性率６８．６％，其中血清分型阳性率为７２．９％。血清１型与基因Ⅱ（１ｂ）型相对应；血清２型与基因Ⅲ（２ａ）型相对应。结论本方法操作简便、快速，具有较强特异性，基本能满足国内临床需要，对丙型肝炎发病及流行病学研究具有重要意义。Objective For finding out the most suitable method for typing hepatitis C cases in our country. Methods The synthetic peptides deduced from the putative Core and NS4 region were used to set up the ELISA system for dividing the HCV antibodies into two types.With this method,we detected the samples from blood donors and chronic hepatitis patients.The antibodies to HCV were tested with Abbott IMx kits and the positive samples were then subjected to serotyping.We detected the genotypes of HCV by polymerase chain reaction with typespecific primers.The relationship between the serotype and genotype was analyzed.Results For blood donors,the positive rates of HCV RNA and anti HCV were 30% and 68.6%,respectively, among the latter 72.9% could be serotypic positive. The serotype 1 corresponded to genotype Ⅱ(1b) and the sreotype 2 corresponded to genotype Ⅲ (2a).Conclusion This ELISA method for identifing serotypes is simple, rapid and with high specificity.It has important significance in clinical,etiological and epidemiological studies of hepatitis C.

关 键 词：丙型肝炎病毒 血清型 合成寡肽 丙型肝炎 ELISA 

分 类 号：R512.630.2[医药卫生—内科学] R373.21[医药卫生—临床医学]