HPLC测定红药片中三七皂苷R1和人参皂苷Rg1的含量  

Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Hongyao Tablet by HPLC

作　　者：卢敏 陈保汉 

机构地区：[1]浙江永宁药业股份有限公司,浙江台州318020

出　　处：《中国现代应用药学》2009年第3期215-217,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立红药片中三七皂苷R1和人参皂苷Rg1的HPLC测定方法。方法采用Agilent1100 DAD高效液相色谱仪,Discovery C18色谱柱(25cm×4.6mm,5μm),乙腈-0.1%磷酸(20∶80)为流动相,检测波长为203nm,流速1mL.min-1。结果三七皂苷R1在1.216～9.728μg(r=0.9995),人参皂苷Rg1在3.036～15.180μg(r=0.9991)内线性关系良好。三七皂苷R1平均回收率为98.6%,RSD=1.7%;人参皂苷Rg1平均回收率为101.0%,RSD=1.7%。结论该法简便、快速、准确,适用于红药片中三七皂苷R1和人参皂苷Rg1的含量定量测定,有利于控制红药片质量。OBJECTIVE To establish a HPLC method for simulataneous determination of Notoginsenoside R1 and Ginsenoside Rgl in Hongyao Tablets. METHODS The HPLC was conducted on Discovery C18 column（25 cm ×4.6 mm,5 μm）witb acetonitrile- 0.1% phosphoric acid solution （20： 80） as the mobile phase. The detection wavelength was 203 nm,and flow rate was 1 mL . min^-1. RESULTS There were good linear relations of Notoginsenoside R1 within 1.216 - 9. 728 μg . mL^-1 （ r = 0. 999 5 ） and Ginsenoside Rgl within 3. 036 - 15. 180 μg . mL^-1 （r = 0. 9991 ）. The average recovery rate of Notoginsenoside R1 was 98.6% , RSD = 1.7% ; and Ginsenoside Rgl was 101.0% ,RSD = 1.7%. CONCLUSION The method is simple, rapid and accurate for the determination of Notoginsenoside R1 and Ginsenoside Rgl in Hongyao Tablets,and it is expected for the quality control of Hongyao tablets.

关 键 词：红药片 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]