日本血吸虫中国大陆株基因重组抗原 Sj22.6kDa 的免疫学活性鉴定  

RESEARCHES ON IMMUNOGENICITY IDENTIFICATION OF RECOMBINANT Sj 22.6(r Sj 22.6) kDa ANTIGEN GENE OF SCHISTOSOMA JAPONICUM 

作　　者：张桂筠[1] 张兆松[1] 陈淑贞[1] 沈一平[1] 吴海玮[1] 苏川[1] 吴观陵[1] 

机构地区：[1]南京医科大学寄生虫学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》1998年第1期6-10,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国务院总理专项研究基金

摘　　要：目的：对重组Ｓｊ２２．６ｋＤａ抗原（ｒＳｊ２２．６）进行免疫学活性鉴定，了解其作为疫苗候选抗原的潜力。方法：Ｗｅｓｔｅｒｎｂｌｏｔ反应确定ｒＳｊ２２．６的抗原性；将ｒＳｊ２２．６注射家兔，制备特异抗血清；以血吸虫尾蚴对Ｃ５７小鼠进行攻击感染，初步鉴定其免疫保护力。结果：ｒＳｊ２２．６可与特异性抗体发生反应，并刺激家兔产生特异性抗体应答，抗体滴度为１１２８０。攻击感染中，免疫组小鼠的减虫率达７７．２％。结论：ｒＳｊ２２．６具有良好的免疫学活性，在诱导抗日本血吸虫感染方面具有一定的潜力。AIM:To identify the immunogenicity and the potentiality of r Sj 22.6 as a candidate vaccine antigen for schistosomiasis japonica. METHODS:r Sj 22.6 was tested by Western blot with specific antibodies to identify its immunogenicity.The r Sj 22.6 protein was isolated and purified by SDS PAGE and injected twice into rabbits to raise antir Sj 22.6 serum that was tested by Western blot to recognize the native protein of adult S.japonicum and SEA.C57 mice were immunized with r Sj 22.6 and challenged with S.japonicum cercariae to identify the protective immunity raised by r Sj 22.6.RESULTS: r Sj 22.6 could be recognized by specific antibodies and could stimulate the rabbits to produce high level of anti r Sj 22.6 antibodies. Anti r Sj 22.6 serum could recognize an adult native protein with the same molecular weight as r Sj 22.6.The preliminary immune challenge test resulted in significant reduction in adult worm burden. CONCLUSION: r Sj 22.6 possesses immunogenicity in stimulating rabbits to produce high level of specific antibody and has protective role as a candidate vaccine antigen against S.japonicum infection.

关 键 词：日本血吸虫 重组抗原 免疫学活性 中国大陆株 

分 类 号：R383.24[医药卫生—医学寄生虫学]