融合蛋白抗原检测不同病程及不同类型囊虫病  

DETECTION OF DIFFERENT PERIODS AND TYPES OF CYSTICERCOSIS BY USING FUSION PROTEIN ANTIGEN

作　　者：王敏[1,2,3] 徐之杰[1,2,3] 孙树汉 郭瀛军[1,2,3] 王俊霞 

机构地区：[1]哈尔滨医科大学寄生虫学教研室 [2]上海第二军医大学分子遗传学教研室 [3]河北医学院分子生物学研究室

出　　处：《寄生虫与医学昆虫学报》1998年第1期19-23,共5页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：猪囊尾蚴四个阳性克隆：２８ＫＤａ、１８ＫＤａ、１４ＫＤａ、３４ＫＤａ以等比联合使用，经异丙基硫代－β－Ｄ半乳糖苷（ＩＰＴＧ）诱导出多克隆β－半乳糖苷酶—猪囊尾蚴重组基因融合蛋白（ＦＰ）抗原，以简化—Ｗｅｓｔｅｒｎｂｌｏｔ（ＳＷＢ）法检测不同病程及不同类型囊虫病，并与粗制抗原（ＣＡ）进行的ＥＬＩＳＡ、ＩＨＡ法相对比。结果显示：用多克隆ＦＰ—ＳＷＢ可提高检出率，且识别抗体更灵敏，阳性率＞８０％，囊虫病人血清循环抗体至少３年中仍有相当高的检出率（＞６０％）。The four positive clones of Cysticercus cellulose:28KDa,18KDa,14KDa and 34KDa in equal proportion induced by isopropylthiogalactose(IPTG) to express multiclone β galactosidase Cysticercus cellulose recombinant FP,using the FP we tested different period and type of Cysticercosis with simplified Western blot (SWB).The tests of crude antigen(CA) ELISA、CA-IHA were evaluated comparatively.The results showed that:tests with multiclone FP SWB can increase the positive rate,the positive rate>80%,multiclone FP SWB is more sensitive to antibody,and the circulating antibody of Cysticercosis patients can maintain at least 3 years(>60%).

关 键 词：囊尾蚴病 融合蛋白 病程类型 诊断 

分 类 号：R532.330.4[医药卫生—内科学]