帕珠沙星与DNA相互作用的光谱研究  

Spectroscopic studies on interaction between pazufloxacin and DNA

作　　者：黄琦[1] 赖依峰[1] 钟文英[2] 

机构地区：[1]浙江科技学院生物与化学工程学院,杭州310023 [2]中国药科大学分析化学教研室,南京210009

出　　处：《中国新药杂志》2009年第6期541-544,共4页Chinese Journal of New Drugs

基　　金：浙江科技学院科研基金重点项目(200603)

摘　　要：目的:研究帕珠沙星与DNA的分子作用机制。方法:利用荧光光谱法研究帕珠沙星与DNA的作用强度并计算热力学数据ΔH;利用紫外光谱、黏度测定、竞争实验、与变性DNA作用的比较等方法确定帕珠沙星与小牛胸腺DNA的相互作用方式。结果:DNA对帕珠沙星的荧光猝灭常数为(9.86±0.39)×103L.mol-1,ΔH=-1156 J.mol-1。结论:帕珠沙星以沟槽键合方式与DNA相互作用。Objective：To study the binding mode of pazufloxacin with DNA. Methods：Fluorescent spectroscopy was used to study the interaction of pazufloxacin with DNA and to calculate the thermodynamic constants. The binding mode was identified by UV-Vis spectra. DNA viscosity titration,competition experiment and the effect of dsDNA and ssDNA on the fluorescense intensity. Results：Pazufloxacin interacted with CT-DNA. The quenching constant was （9.86 ±0.39） × 10^3 L.mol^-1. The interaction was exothermic with a Van＇t Hoff enthalply of -1 156 J.mol^-1. Conclusion：Pazufloxacin interacts with CT-DNA in the mode of groove binding.

关 键 词：帕珠沙星 小牛胸腺DNA 沟槽键合 

分 类 号：R917[医药卫生—药物分析学] R978.26[医药卫生—药学]