ω-3多不饱和脂肪酸对去卵巢大鼠骨生物力学的影响  

Effects of ω-3 polyunsaturated fatty acids on bone biomechanics in ovariectomized rats

作　　者：许萍[1] 马戎[2] 常小霞[3] 吴洁[3] 翟青新[4] 沈小志[1] 刘宝瑞[5] 

机构地区：[1]南京大学医学院附属鼓楼医院营养科,江苏南京210008 [2]南京大学医学院附属鼓楼医院普通外科,江苏南京210008 [3]江苏省人民医院妇女保健科,江苏南京210036 [4]南京大学医学院附属鼓楼医院动物实验中心,江苏南京210008 [5]南京大学医学院附属鼓楼医院肿瘤科,江苏南京210008

出　　处：《肠外与肠内营养》2009年第2期96-99,共4页Parenteral & Enteral Nutrition

基　　金：南京市卫生局科研课题资助(YKK05124;YKK05101)

摘　　要：目的:探讨ω-3多不饱和脂肪酸(ω-3 PUFA)治疗对去卵巢大鼠骨生物力学的影响。方法:将60只雌性SD大鼠手术切除双侧卵巢或等体积脂肪,分为去卵巢(OVX)组、17α-炔雌醇(E2)组、小剂量ω-3 PUFA(A组)、中剂量ω-3 PUFA(B组)和大剂量ω-3 PUFA(C组),并设假手术(Sham)组为对照。用药90 d后,分离大鼠股骨,用分析天平测骨量,三点弯曲实验法测最大载荷、最大挠度、弹性载荷、弹性挠度和弹性能量吸收。结果:大鼠去卵巢后股骨结构力学特性指标与Sham组比均显著下降,各用药组与OVX组相比均有显著上升。结论:ω-3PUFA能改善去卵巢大鼠骨的生物力学性能,降低其发生骨折的危险性。Objective： To explore the benefit effects of ω-3 polyunsaturated fatty acids on bone biomechanics in ovarieetomized （OVX） rats. Methods： 60 virgin female Sprague-Dawley rats were randomized by the stratified weight method into 6 groups： ① Sham group： deionized water 2 mL/d; ②OVX group：deionized water 2 mL/d; ③E2 group： OVX ＋ 17α- ethinylestradiol 0. 1 mg/（kg · d）;④A group： OVX ＋ω-3 PUFAs 75 mg/（kg· d） ; ⑤B group： OVX ＋ ω-3 PUFAs 150 mg/（kg · d） ; ⑥ C group ： OVX ＋ ω-3 PUFAs 300 mg/（kg · d）. Each group had 10 rats. After feeding for 90 days, the rats were killed and femur were separated. The maximum load, maximum deformation, elastic load, elastic deformation and energy absorption were measured from three point bending test. Bone wet weights were measured. Result： The femur biomechanics markers of OVX group decreased obviously, while that of all medicated group increased obviously（ P 〈 0.01 ）. Conclusion ：ω-3 PUFAs have benefit effects on bone biomechanics property and decrease the risk of the bone fracture.

关 键 词：Ω-3多不饱和脂肪酸 去卵巢大鼠 骨生物力学 骨质疏松 

分 类 号：R580[医药卫生—内分泌]