金黄色葡萄球菌L型感染和k-ras基因点突变在卵巢癌发生发展中的相关性  

Correlation of Staphylococcus Aureus L-form Infection and Mutation of k-ras in Ovarian Carcinomas

作　　者：俞岚[1] 汪万英[2] 甘怀勇[1] 

机构地区：[1]蚌埠医学院病理学教研室感染与免疫重点实验室,安徽蚌埠233030 [2]蚌埠医学院附属医院中心实验室,安徽蚌埠233004

出　　处：《癌变．畸变．突变》2009年第2期98-101,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：蚌埠医学院科研项目(BY0813)

摘　　要：背景与目的:探讨金葡菌L型感染和k-ras基因点突变在卵巢癌发生发展中的相关性。材料与方法:应用革兰染色、PCR-FLRP分析技术检测97例卵巢乳头状癌、23例卵巢乳头状瘤石蜡包埋组织中金葡菌L型的感染率及k-ras基因点突变情况。结果:卵巢乳头状癌组织中伴有金葡菌L型感染的有25例,检出率为25.8%(25/97),该组k-ras基因密码子12突变率为45.4%;卵巢乳头状瘤组织中伴有金葡菌L型感染的有3例,检出率为13.0%(3/23),该组k-ras基因密码子12突变率为8.7%。金葡菌L型感染与k-ras基因点突变的关系,经Spearman等级相关分析,乳头状癌组为r=0.5047,P<0.05,乳头状瘤组为r=-0.1195,P>0.05。结论:卵巢乳头状癌组织中金葡菌L型感染与k-ras基因点突变具有正相关关系,而乳头状瘤则不具有相关性。BACKGROUND AND AIM：To explore the correlation of staphylococcus aureus L-form infection and mutation of k-ras in ovarian carcinomas.MATERIALS AND METHODS：Gram staining and PCR-FLRP were used for detection of bacterial L-forms in paraffin-embeded tissues of 97 ovarian papillary carcinomas and 23 benign tumors.RESULTS：The positive rate of L-forms in ovarian carcinomas was 25.8%（25/97）,and in this group,the mutation rate of k-ras at codon 12 was 45.5%.However the positive rate of L-forms in ovarian benign tumors was only 13.0%（3/23）,and in this group,the mutation rate of k-ras at codon 12 was only 8.7%.With Spearman analysis,the malignant group was r=0.504 7（P〈0.05）;and the benign group was r=-0.119 5（P〉0.05）.CONCLUSION：Infection of L-forms had a positive correlation with point mutation of k-ras in malignant tumors but not in benign tumors.

关 键 词：卵巢肿瘤 金黄色葡萄球菌L型 聚合酶链反应 限制性片段长度多态性 

分 类 号：R737.31[医药卫生—肿瘤]