Characterization of Bacterial Strains Isolated from a Novel Seawater-based Retting Treatment of Hemp  

作　　者：朱润晔 陈建勇 冯新星 张建春 

机构地区：[1]College of Biological and Environmental Engineering,Zhejiang University of Technology [2]Key Laboratory of Advanced Textile Material and Manufacturing Technology,Ministry of Education,College of Materials and Textiles,Zhejiang Sci-Tech University [3]The Research Center of China-hemp Materials,The Quartermaster Research Institute of the General Logistic Department of CPLA

出　　处：《Journal of Donghua University(English Edition)》2008年第6期686-693,共8页东华大学学报（英文版）

基　　金：Programfor Changjiang Scholars and Innovative Research Teamin University,China( No .IRT0654);Major State Basic Research Development Program,China ( No .2008CB617506);Analysis and Test Foundation of Zhejiang Province,China ( No .2007F70040)

摘　　要：Cultivable bacteria were isolated from seawater-based retting treatment of hemp, in which three of purified strains (SW- 1, SW- 2, and S-SW1) produced relatively high levels of pectinase activities, and also produced mannanases and xylanases. PCR-based entebacterial repetitive intergenic consensus primers (ERIC-PCR) were employed for fingerprinting DNA of the bacterial strains. The ERIC-PCR fingerprints of stains SW- 1, SW- 2, and S-SW1 were found to be different, and should be further identified for each isolate. Strains SW- 1 and SW- 2 were identified as Stenotrophomnas maltophilia, while strain S-SW1 was assigned to Ochrobactrum anthropi by BIOLOG system. These two species represented rhizosphere bacterial genera, and possibly were introduced by the hemp plants. These organisms seemed potentially capable of producing pectinase and hemicellulase, and thus effectively degrading the gum substances in the seawater retting. This research could be helpful for improving a novel seawater-based retting treatment of hemp.Cultivable bacteria were isolated from seawater-based retting treatment of hemp, in which three of purified strains （SW- 1, SW- 2, and S-SW1） produced relatively high levels of pectinase activities, and also produced mannanases and xylanases. PCR-based entebacterial repetitive intergenic consensus primers （ERIC-PCR） were employed for fingerprinting DNA of the bacterial strains. The ERIC-PCR fingerprints of stains SW- 1, SW- 2, and S-SW1 were found to be different, and should be further identified for each isolate. Strains SW- 1 and SW- 2 were identified as Stenotrophomnas maltophilia, while strain S-SW1 was assigned to Ochrobactrum anthropi by BIOLOG system. These two species represented rhizosphere bacterial genera, and possibly were introduced by the hemp plants. These organisms seemed potentially capable of producing pectinase and hemicellulase, and thus effectively degrading the gum substances in the seawater retting. This research could be helpful for improving a novel seawater-based retting treatment of hemp.

关 键 词：seawater retting strain identi fication BIOLOG system ERIC-PCR 

分 类 号：S852.659.5[农业科学—基础兽医学] TQ458[农业科学—兽医学]