生长因子对人视网膜神经胶质细胞增殖的作用  

Growth factor effects on the proliferation of human retinal glial cell in culture

作　　者：蔡季平[1] 周韵秋[1] 奚寿增[1] 

机构地区：[1]第二军医大学长征医院眼科

出　　处：《中华眼底病杂志》1998年第1期33-34,共2页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的研究生长因子对培养的人视网膜神经胶质细胞增殖的作用。方法在培养的人视网膜神经胶质细胞中分别加入表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ，０．５～１００．０ｎｇ／ｍｌ）和神经生长因子（ｎｅｒｖｅｇｒｏｗｔｈｆａｃｔｏｒ，ＮＧＦ，０．０５～１０．０ｎｇ／ｍｌ），培养３天后用ＭＴＴ法测量细胞增殖情况。结果ＥＧＦ（０．５～１００．０ｎｇ／ｍｌ）和ＮＧＦ（０．０５～１０．０ｎｇ／ｍｌ）均能刺激人视网膜神经胶质细胞的增殖，其ＥＣ５０（ｈａｌｆｅｆｅｃｔｉｖｅｃｏｎｃｅｎｔｒａｔｉｏｎ，半数有效浓度）分别为１７．０ｎｇ／ｍｌ和０．７ｎｇ／ｍｌ。结论ＥＧＦ和ＮＧＦ均能刺激人视网膜神经胶质细胞的增殖，它们可能在增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅ－ｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）等增殖性病变中起一定作用。Objective To study the effects of growth factor on the proliferation of the cultured human retinal glial cells. Methods EGF(0.5～100.0ng/ml)and NGF(0.5～10.0ng/ml) were added to cultures of human retinal glial cells and the proliferation rates of the cells were measured by MTT method. Results EGF at a dosage ranging from 0.5ng/ml to 100.0ng/ml and NGF (0.05～10.0ng/ml) stimulated the cellular proliferation effectively with their EC 50 of 17ng/ml and 0.7 ng/ml respectively. Conclusion Both EG Fand NGF had an effective stimulation on human retinal glial cell proliferation.They may play a role in the formation of PVR.

关 键 词：表皮生长因子 尿抑胃素 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] Q516[医药卫生—基础医学]