水体中添加两种菌剂对凡纳滨对虾存活、生长及消化酶活力的影响  

Effect of adding two microbes to aquaculture water on shrimps' survival,growth and digestive enzyme activity

作　　者：宋奔奔[1,2] 傅松哲[3,2] 刘志培[3] 石芳永[4] 刘鹰[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100049 [3]中国科学院微生物研究所,北京100101 [4]青岛理工大学,山东青岛266033

出　　处：《海洋科学》2009年第4期1-5,共5页Marine Sciences

基　　金：国家自然科学基金项目(30671620);国家科技支撑计划项目(2006BAD09A03);国家863计划项目(2006AA100305)

摘　　要：向水体中添加不同浓度的短小芽孢杆菌CGMCC 1004(Bacillus pumilus)和胶红酵母菌CGMCC1013(Rhodotorula mucilaginosa)以研究凡纳滨对虾(Litoenaeus vannamei)体长、体质量、存活率、胃蛋白酶、肝胰腺淀粉酶和肝胰腺脂肪酶的影响,以及需钠弧菌(Vibrio natriegens)的感染效果。结果表明:向水体中添加104CFU/mL短小芽孢杆菌能提高对虾体长、存活率,但结果不显著,但对体质量增长率具有显著提高作用;水体中添加104CFU/mL短小芽孢杆菌对提高需钠弧菌感染后对虾成活率有一定的提高作用;水体中添加104CFU/mL短小芽孢杆菌能显著提高胃蛋白酶活性,但是对肝胰腺淀粉酶活性有一定抑制作用;添加复合菌剂比添加单一菌种的作用要好;亦证明了向水体中添加细菌的方式对凡纳滨对虾的体长、体质量、存活率以及水质指标影响比较小,但是对消化酶具有一定的提高作用。Bacillus purnilus and Rhodotorula rnucilaginosa with different concentrations were added to aquaculture tanks for shrimp（Litopenaeus vannamei）, and such parameters as body length, body weight, survival rate, stomach proteinase, hepatobiliary ＆ pancreatic amylase, hepatobiliary ＆ pancreatic lipase of shrimp, et al. were determinated. An experimental infection of shrimp by Vibrio natriegens was also tested. The results showed that a treatment group with 100 mg/kg Bacillus pumilus could promote body length and livability, but there is no significant difference. And this group can significantly promote body weight. This group could improve shrimp livability after the infection of Vibrio natriegens, significantly enhanced a stomach proteinase activity, but inhibited the activity of the hepatobiliary ＆ pancreatic amylase; the treat- ment group with two species is better than the group with one in inhibiting the activity of hepatohiliary. And the results showed that methodology of adding bacteria to waters has few effect on shrimp body length, bodyweight, livability and water quality, but can improve digestiveenzyme activities.

关 键 词：凡纳滨对虾(Litopenaeus vannamei) 消化酶 短小芽孢杆菌(Bacillus pumilus) 胶红酵母菌(Rhodotorula mucilaginosa) 需钠弧菌(Vibrio natriegens) 

分 类 号：S917.4[农业科学—水产科学]