Sec-10影响神经肌肉接头突触后敏感性的研究(英文)  

Sec-10 Knockout Increases The Neuroactive-drug Responses Without Affecting Function of The Postsynaptic Ionotropic Receptors in Neuromuscular Junctions

作　　者：章雷[1] 李江丽[1] 高尚邦[1] 吴政星[1] 张蓉颖[1] 徐涛[1,2] 

机构地区：[1]华中科技大学分子生物物理教育部重点实验室,武汉430074 [2]中国科学院生物物理研究所,生物大分子国家重点实验室,北京100101

出　　处：《生物化学与生物物理进展》2009年第4期410-416,共7页Progress In Biochemistry and Biophysics

基　　金：This work was supported by a grant from The National Science Foundation of China (30500117, 30770523)~~

摘　　要：exocyst复合物在分泌过程中起重要作用.可是分子机理尚未研究透彻.通过TMP/UV的基因敲除方法,得到了exocyst复合物中一种组分sec-10的缺陷型线虫.药学结果显示这种线虫神经信号传递存在缺陷,可是电生理的方法证明在神经肌肉接头处的已报道离子型受体和野生型相比并未发生改变.因此猜测,sec-10并未直接影响神经肌肉接头处的离子型受体,而是通过其他途径来行使功能.Exocyst complex is known to function in the exocytosis network, however, the molecular mechanism is unclear yet. UsingUV/trimethylpsoralen mutagenesis, the sec-10 （one component of the exocyst complex） knockout mutant of C. elegans was obtained for the first time. The drug sensitive assays revealed clearly that the sec-lO gene affected the neural signal transmission, however, the electrophysiological assay showed the function of the ionotropic receptors in the neuromuscular junctions （NMJs） were unaltered compared with the wild type （WT）. Thus it was assumed that the sec-10 gene might not influence the known ionotropic receptors in the NMJs, but some other pathways instead.

关 键 词：exocyst复合物 sec-10 神经肌肉接头 离子型受体 

分 类 号：Q71[生物学—分子生物学] Q424