西瓜核雄性不育两用系G17AB育性基因的AFLP分子标记  

Identification of AFLP Markers Linked to the Genic Male Sterility Trait in Watermelon

作　　者：郭守鹏[1] 刘海河[1,2] 张彦萍[2] 谢彬[1] 张成合[1] 申书兴[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北工程大学农学院,河北邯郸056021

出　　处：《园艺学报》2009年第3期427-430,共4页Acta Horticulturae Sinica

基　　金：河北省自然科学基金项目(C2006000616)

摘　　要：利用AFLP标记技术,结合BSA法,用256个EcoRⅠ/MseⅠ(3+3)引物组合对西瓜核雄性不育两用系G17AB进行了分析,得到了一条特异的扩增条带E31M50。用该引物对后代分离群体进行分析,确定了E31M50与育性基因的遗传距离为5.0cM。通过对这个多态性特异片段进行回收和测序,得到了这个337bp片段的全长序列。该标记可以用于分子辅助育种和不育株早期筛选。The genic male-sterile and male-fertile lines were compared by using 256 primers [EcoRⅠ/MseⅠ（3＋3）] with the AFLP technique.A specific amplified DNA fragment,E31M50,that linked to the pollen fertility was identified from watermelon.The genetic distance between E31M50 and the fertility gene is 5.0 cM.The 337 bp E31M50 segment was recovered and sequenced.The marker could potentially be used for breeding and gene identification.

关 键 词：西瓜 雄性不育基因 AFLP标记 

分 类 号：S651[农业科学—果树学]