黄芪皂苷抑制HEK293sw细胞Aβ生成的机制研究  

Mechanism of the inhibiting effect of AST on Aβ production in HEK293sw cells

作　　者：张瑞[1] 王子玫[1] 夏宗勤[1] 胡雅儿[1] 

机构地区：[1]上海交通大学医学院细胞调控研究室,上海200025

出　　处：《中国新药杂志》2009年第5期441-444,共4页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30070926);上海市高校选拔培养优秀青年教师科研专项基金

摘　　要：目的:探讨黄芪皂苷(AST)抑制HEK293sw细胞-淀粉样肽(Aβ)生成的作用机制。方法:采用HEK293sw细胞,常规培养后加入不同浓度(0,0.1,1和10μmo.lL-1)AST处理,荧光法测定β-分泌酶活力,并用Western blot和RT-PCR技术检测淀粉样前体蛋白(APP)和β-位点APP切割酶1(BACE1)的表达。结果:在HEK293sw细胞模型上,AST终浓度1和10μmol.L-1显著降低β-分泌酶活力。1和10μmol.L-1AST对APP的蛋白和mRNA表达无影响,但对BACE1的蛋白水平都有降低作用,同时也显著降低mRNA的水平。结论:AST可能通过抑制β-分泌酶而降低Aβ的产生。Objective： To investigate the mechanism of the inhibiting effect of AST on β-amyloid peptide（Aβ） production in HEK293sw cells.Methods： Routinely cultured HEK293sw cells were treated by different concentrations of AST.At 72h later,cells were examined for β-secretase activity by fluorescence method with a kit supplied by R＆D Systems,and were examined for amyloid precursor protein（APP） and β site APP cleaving enzyme 1（BACE1） protein expressions by Western blot and for mRNAs with RT-PCR.Results： At 1 and 10μmol·L^-1,AST significantly decreased β-secretase activity,had no effect on protein or mRNA expression of APP,but decreased BACE1 protein and mRNA level in a concentration-dependent manner.Conclusion： AST can decrease the expression of Aβ protein through the inhibition of β-secretase in HEK293sw cells.

关 键 词：黄芪皂苷 Β-淀粉样肽 淀粉样前体蛋白 Β-分泌酶 β-位点APP切割酶1 

分 类 号：R285.5[医药卫生—中药学] R971.91[医药卫生—中医学]