隐孢子虫体外培养研究进展  

ADVANCE ON IN VITRO CULTIVATION OF CRYPTOSPORIDIUM SPP.

作　　者：张素梅[1] 赵金凤[1] 张龙现[1] 宁长申[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《寄生虫与医学昆虫学报》2009年第1期57-63,共7页Acta Parasitologica et Medica Entomologica Sinica

基　　金：河南省高校创新人才培养工程项目（豫教高[2004]294号）

摘　　要：隐孢子虫是一种重要的人兽共患寄生性原虫,可引起儿童和免疫低下人群以及幼龄动物发生严重胃肠道疾病。长期以来围绕建立隐孢子虫体外培养模型国外学者做了大量探索,国内则在该领域尝试不多。为了研究隐孢子虫详细的内生发育过程,快速筛选抗隐孢子虫的有效药物、研究功能基因表达和外源基因的有效表达等,需要建立有效的体外培养模型。本文就20多年来国内外隐孢子虫培养概况进行了综述。Cryptosporidium is an intracellular parasitic protozoan that can cause severe disease in a wide variety of young animals, children and immunocompromised persons. In vitro cultivation is sufficient to support a variety of studies, including characterizing life cycle stage, assessing potential drug therapies, evaluating oocyst disinfection methods, and gene expression. The attempts and significant progress made in the past over 20 years, were reviewed in present paper for making great strides in producing in vitro culture models for Cryptosporidium parasites.

关 键 词：隐孢子虫 种类 内生发育 细胞培养 鸡胚培养 

分 类 号：Q813.11[生物学—生物工程] S852.723[农业科学—基础兽医学]