温度对浸矿微生物活性及铜浸出率的影响  

Effect of temperature on the activity of mineral-bioleaching microorganisms and the bioleaching rate of copper

作　　者：温建康[1] 姚国成[1] 陈勃伟[1] 黄松涛[1] 

机构地区：[1]北京有色金属研究总院生物冶金国家工程实验室,北京100088

出　　处：《北京科技大学学报》2009年第3期295-299,共5页Journal of University of Science and Technology Beijing

基　　金：国家重点基础研究发展计划资助项目(No.2004CB619205);国家高技术研究发展计划资助项目(No.2007AA060901)

摘　　要：研究了从紫金山铜矿生物堆浸矿堆中分离出来的浸矿菌群在不同温度下的氧化活性和对紫金山硫化铜矿石的浸出规律,并利用变性梯度凝胶电泳法研究了15℃和30℃的菌群组成情况.结果表明:所分离的细菌的最佳生长温度为30℃,浸出10d,铜浸出率为87.62%,在最佳生长温度以外铜浸出率都会降低;15℃和30℃的优势菌分别为Leptospirillum fer-riphilum和Acidithiobacillus thiooxidans,两个温度下均有同一种劣势菌Acidithiobacillus caldus;优势菌种随温度的变化是与菌种的性质相关的.Bioleaching test of Ziiinshan copper sulfide ore in a shake flask was conducted at different temperatures. Bacteria culture from Zijinshan copper mine bioheap was applied in this test. The highest copper leaching rate of 87.62 % in 10 d was achieved at 30 ℃. Microbial community at 15 ℃ and 30 ℃ was analyzed by denaturing gradient gel electrophoresis. The dominant bacteria groups at 15℃ and 30 ℃ are Leptospirillum ferriphilum and Acidithiobacillus thiooxidans respectively, together with Acidithiobacillus caldus accounting high proportion at both the temperatures. The change in microbial community with temperature is correlated with its characteristics.

关 键 词：生物浸出 微生物活性 变性梯度凝胶电泳 浸矿微生物 

分 类 号：TF18[冶金工程—冶金物理化学]