小麦拔节过程中差异表达基因WSR1的克隆及序列分析  

Cloning and Sequence Analysis of A Differentially Expressed Gene WSR1 in the Elongating Stems of Wheat

作　　者：王创云[1,2] 王美霞[1,2] 卢成达[1,2] 袁文业[1,2] 张丽娜[1,2] 

机构地区：[1]山西腾达种业有限公司,山西太原030031 [2]山西省农业科学院作物遗传研究所,山西太原030031

出　　处：《山西农业科学》2009年第3期22-24,共3页Journal of Shanxi Agricultural Sciences

基　　金：山西省国际科技合作项目(2008081007);太原市技术创新计划项目(0801009);山西省农业科学院青年基金项目(YQN0602)

摘　　要：为了探讨小麦茎节伸长的分子机理,在DDRT-PCR技术分析小麦拔节过程中的基因差异表达谱基础上,克隆了一个在拔节过程中沉默表达的基因,命名为WSR1。对该基因cDNA片段的序列和生物信息学分析显示,WSR1为Ser/Thr蛋白激酶基因,其编码蛋白与大麦中编码Ser/Thr蛋白激酶的序列具有68%的同源性。通过半定量RT-PCR技术分析显示,该基因在小麦拔节过程中的表达模式与通过DD-RT-PCR分析所展示的差异表达趋势基本上一致。To study the molecular mechanism of wheat stem elongation, the differential display reverse transcription PCR （DDRT-PCR） was used to identify the gene expression pattern during the basal stem elongation in wheat plant. On the basis of gene different expression profiles, one cDNA which observed in pre-elongation basal stem tissue, but not in elongated was cloned, named WSR1. Sequence analysis and homology search in GenBank database showed that WSR1 had 68% similarity to barley serine/threonine protein kinase; The expression patterns of the cloned cDNAs were analyzed via semi-quantitative RT-PCR in the elongating wheat stem, the results showed that the expression patterns was basically agree with the expression trends displayed in DDRT-PCR analysis.

关 键 词：小麦 茎节伸长 基因差异表达 

分 类 号：S512[农业科学—作物学] Q785[生物学—分子生物学]