鸡骨草提取物对体外乙型肝炎病毒的抑制作用  

The Inhibitory Effect of Abrus Cantoniensis Hance on Hepatitis B Virus in Vitro

作　　者：陈晓白[1] 韩余健 许潘健[2] 

机构地区：[1]广西壮族自治区玉林师范学院化生系,537000 [2]广西壮族自治区玉林市红十字会医院检验科,537000

出　　处：《医药导报》2009年第4期418-420,共3页Herald of Medicine

基　　金：广西教育厅科研项目(基金编号:200710MS052)

摘　　要：目的观察鸡骨草提取物对体外HBsAg和HBeAg的抑制作用。方法将鸡骨草醇提取液与等容量、滴度为1:64的HBsAg和HBeAg阳性血清混合,使混合后鸡骨草的浓度分别为96,48,24,12和6g.L-1,用磷酸缓冲液与1:64的HBsAg和HBeAg阳性血清混合作空白对照。每组6管,37℃水浴8和16h后,采用ELISA法测定HBsAg和HBeAg的吸光度(A值),观察其对HBsAg或HBeAg的抑制作用。结果鸡骨草醇提取液对血清中HBsAg和HBeAg均具有明显的抑制作用,其中鸡骨草提取物在浓度为96,48g.L-1时对HBsAg、HBeAg抑制作用最为明显(P<0.05或P<0.01)。结论鸡骨草提取物在体外有较明显的抗HBsAg和HBeAg作用。Objective To observe the inhibitory effect of Abrus cantoniensis Hance on HBsAg and HBeAg in vitro. Methods The ethanol extracts of Abrus cantoniensis Hance was mixed with HBsAg and HBeAg masculine serum which had same capacity and same titer（ the titer of 1 ： 64 ）. After mixing, the densities of Abrus cantoniensis Hance were 96,48,24,12 and 6 g · L^-1 ,respectively. And PBS with masculine serum （the titer of 1 ： 64） was made as blank control. The optical density （A）of HBsAg and HBeAg in each serum sample were determined by ELISA after 8 h and 16 h＇s cultivation in water at 37℃ Results The ethanol extracts of Abrus cantoniensis Hance had obvious inhibitory effect on HBsAg and the HBeAg in serum at the density of 96 g · L^-1 and 48 g · L^-1 （ P 〈 0.05 or P 〈 0.01 ）, respectively. Conclusion Abrus cantoniensis Hance could inhibit obviously HBsAg and HBeAg in vitro.

关 键 词：鸡骨草 HBSAG HBEAG 抑制 

分 类 号：R285[医药卫生—中药学]