大蕉胚性培养物及其原生质体再生植株的染色体分析  

Chromosome Number of Embryogenic Cultures and Protoplast-regenerated Plants of Musa paradisiaca Linn. cv. Da Jiao,ABB

作　　者：肖望[1,2] 黄少丽[2] 陈晓娜[2] 戴雪梅[1] 黄霞[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广东教育学院生物系,广东广州510303

出　　处：《中山大学学报（自然科学版）》2009年第2期71-75,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30400287);广东省自然科学基金资助项目(011126,06023159);广东省科技计划资助项目(2006B20101014);广州市科技计划资助项目(2006Z3-E0281)

摘　　要：采用看护培养法对大蕉胚性细胞悬浮系(Embryogenic cell suspensions,ECS)来源的原生质体进行培养,并研究了不同品种的看护细胞对原生质体培养的影响,以及大蕉ECS及其原生质体再生植株的染色体数目。结果发现,采用贡蕉ECS作为看护细胞时,大蕉原生质体培养15 d后细胞分裂频率达到8.5%,培养30 d后细胞团形成率为0.35%;而以大蕉ECS作为看护细胞时,全部原生质体褐化,不能正常发育。染色体检测结果表明,大蕉ECS的染色体数目不稳定,除含有正常的三倍体染色体数目的细胞(2n=3x=33)外,还出现大量染色体数目异常的细胞。其中绝大部分细胞含有多倍和非整倍的染色体数目,仅14.5%的细胞含正常三倍体染色体数目;但通过ECS原生质体培养获得的再生植株具有正常的三倍体染色体数目(2n=3x=33)。Protoplasts isolated from embryogenic cell suspensions of Musa paradisiaca Linn. cv. Da Jiao, ABB were cultured on feeder layer. Then the effect of nurse cells on protoplast culture, the chromosome numbers of embryogeneic suspension cells and protoplast-regenerated plants of Da Jiao （ABB） were studied. When embryogenic cell suspensions of Musa acuminate cv. Mas （AA） were used as nurse cells, the cell division frequency at 14 days and colony formation frequency at 28 days were 8.5% and 0. 35% , respectively. And the protoplasts were necrosis when embryogenic cell suspensions of Da Jiao （ ABB ） were used as nurse cells. The chromosome numbers of embyrogenic cell suspensions of Da Jiao （ABB） showed a wide range of variation. Most of them were aneuploidy and polyploidy, while only about 14. 5% of cells were observed to be triploid （2n = 3x = 33）. But the chromosome number of the protoplast-regenerated plants was triploid （2n =3x =33）.

关 键 词：大蕉Musap aradisiaca Linn. cv. DaJiao ABB 胚性细胞悬浮系(Embryogenic cell suspensions ECS) 再生植株 原生质体培养 染色体数目 

分 类 号：S668.1[农业科学—果树学]