HPLC测定黄芪药渣中黄芪甲苷含量  

Determination of Astragaloside IV in Dreg of Radix astragali by High-Performance Liquid Chromatography

作　　者：黄亚非[1] 刘杰[1] 黄际薇[1] 张永明[1] 

机构地区：[1]中山大学附属第三医院,广东广州610630

出　　处：《中山大学学报（自然科学版）》2009年第2期146-148,共3页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省中医药局科技资助项目(100112)

摘　　要：对内蒙产黄芪药材及药渣采用甲醇梯度洗脱提取，高效液相色谱法测黄芪甲甙的质量分数．回归方程Y=1．6084X＋13．2468，R=0．9997，在5．50～550．00μg／mL之间的线性关系良好。日内和日间精密度的RSD均小于1．7％，重复性试验的RSD为0．59％（n=5）。黄芪药材的加样回收率为96．62％、98．23％、97．46％，RSD（n=5）分别为2．6％、2．8％、3．4％；药渣的加样回收率为98．43％、98．34％、99．65％，RSD（n=5）分别为2．3％、3．1％、2．9％。黄芪药渣中黄芪甲苷的提取量为黄芪药材中黄芪甲苷提取量的72．08％，表明黄芪药渣仍具有较大的再利用价值。The Radix astragali and its dreg were extracted with methanol. The astragaloside IV in Radix astragali and its dreg were determined by High-performance Liquid Chromatography （HPLC）. The calibration curve was linear in the range of S. 50 -550. 00 μg/mL, Y = 1. 680 4X ＋ 13.246 8, R = 0. 999 7. The intra-day and inter-day precision （RSD） were all less 1.7%, the recurrence （RSD） was 0. 59 （n = 5）. The recovery of Radix astragali were 96.62%, 98.23%, 97.46% and RSD （ n = 5） were 2. 6%, 2. 8%, 3.4% for astragaloside IV; The recovery of dreg of Radix astragali dreg were 98.43%, 98.34%, 99. 65%, RSD （n =5） were 2. 3%, 3. 1%, 2. 9% for astragaloside IV. Astragaloside IV in dreg was 72% of that in Radix astragali. The result showed that the dreg of Radix astragali is still great re-utilizable value.

关 键 词：HPLC—ELSD 黄芪药材 黄芪药渣 黄芪甲苷 

分 类 号：R93[医药卫生—生药学]