荧光共轭聚合物在生物大分子检测中的应用  

Application of Fluorescent Conjugated Polymers in Detecting Biomacromolecules

作　　者：支俊格[1] 徐秀玲[1] 申进波[2] 赵玮[2] 佟斌[2] 董宇平[2] 

机构地区：[1]北京理工大学理学院化学系,北京100081 [2]北京理工大学材料科学与工程学院,北京100081

出　　处：《化学进展》2009年第4期739-746,共8页Progress in Chemistry

基　　金：国家自然科学基金项目(No.20504005;20634020)资助

摘　　要：共轭聚合物因其具有π电子体系及共轭离域结构,一般都具有优异的发光性能,其发光强度和发射波长会随被检测化合物结构的不同而发生特异性响应,特别是在与被检测物相互作用过程中产生的电荷和能量能够沿共轭分子链进行有效传递,成倍放大这种作用,从而有效提高了检测灵敏度,这比相应的小分子化合物更具有优越性。目前共轭聚合物已被用于开发新型化学、生物传感器,尤其是在生物分子检测方面的应用得到迅速发展。本文总结了近年来荧光共轭聚合物在生物传感方面的研究进展,主要讨论共轭聚合物在蛋白质、核酸及毒素检测中的应用。Conjugated polymers exhibit excellent electronic and luminescent properties due to their special π- electronic system and conjugated delocalized structure. The fluorescence intensity and emission wavelength of them vary obviously and selectively with different interactions between conjugated polymer and detected compounds. The fluorescence signals deriving from the interactions between conjugated polymer and detected compounds are often amplified because the effective electrons and energy transfer along the main-chain of conjugated polymers, which can respond high- sensitively to analytes and exceed to that of small model compounds. The novel chemical and biological sensors based on conjugated polymers are developed. Many conjugated polymers, including poly（thiophene）s, poly （fluorene-cophenylene） s, poly（ phenylene ethynylene）s and poly（ phenylene vinylene）s are used to detect biomacromolecules. In the present paper, the recent progress of conjugated polymers as biosensors is summarized, and the assay of protein, DNA and toxin using fluorescent conjugated polymers is discussed.

关 键 词：荧光共轭聚合物 生物检测 荧光猝灭 

分 类 号：O657.3[理学—分析化学] O629[理学—化学]