方形黄鼠蚤阿尔泰亚种感染蚤越冬后菌栓形成实验  

The forming of bacterial embolus from the fleas (Citellophilus tesquorum altaicus) infected by Yersina pestis after hibernation

作　　者：林纪春[1] 王诚[1] 张晓雪[1] 冯玉明[1] 雷刚[1] 钱存宁[1] 

机构地区：[1]新疆维吾尔自治区疾病预防控制中心鼠防科,乌鲁木齐830002

出　　处：《中国媒介生物学及控制杂志》2009年第2期159-160,共2页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(30560138)

摘　　要：目的了解方形黄鼠蚤阿尔泰亚种带菌越冬后菌栓形成的客观水平。方法2006年9月28日将饥饿3d的方形黄鼠蚤阿尔泰亚种,置感染鼠疫菌而濒死的小鼠体吸血,剔出未吸血蚤,将感染的方形黄鼠蚤413只置于温度2～4℃,相对湿度75%～85%的冷藏箱中。2007年4月6日将蚤从冷藏箱取出,用小鼠全天喂饲吸血1次。此后置于温度20～22℃,相对湿度75%～85%温箱中,隔日用健康小鼠供血0.5～1h,镜下观察菌栓形成情况。结果经过一个冬眠期共存活235只方形黄鼠蚤,形成菌栓蚤9匹,菌栓率3.83%(9/235)。结论方形黄鼠蚤带菌越冬后菌栓形成率明显低于黄鼠活动期菌栓蚤的形成率(13.8%),这可能与蚤在不利环境下出现滞育现象和一部分蚤已摆脱鼠疫菌或带菌量减少,低于102～104有关。Objective To understand the objective level of the bacterial embolus forming of the fleas （Citellophilus tesquorum altaicus） after its hibernation. Methods C. tesquorum altaicus hungered for 3 d were fed with the dying mice infected by Yersina pestis on September 28, 2006, and no blood-sucking fleas were removed. Four hundred and thirteen infected fleas were put into the deepfreez at 2 to 4 ℃ and relative humidity （RH） 75%-85%. The infected fleas were taken out from refrigerator on April 6, 2007, and sucked blood from non-infected mouse one time. Then they were put into the incubator at 20 to 22 ℃ and RH 75%-85%. They were fed for 0.5-1 h at one day intervals. The forming of bacterial embolus was observed by microscope. Results There were 235 C.tesquorum altaicus alive after hibernation, and nine fleas formed the bacterial embolus, which the forming rate of bacterial embolus was 3.83% （9/235）. Conclusion The forming rate of bacterial embolus of the fleas carrying the plague to over-winter was obviously lower than that of the fleas in the active stage （13.8%）, which might be related to fleas diapause at the disadvantage environment and the situation that some fleas got rid off plague or the plague quantity carried by fleas decreased, less than 10^2 to 10^4.

关 键 词：感染蚤 越冬 菌栓蚤 菌栓率 

分 类 号：R384.3[医药卫生—医学寄生虫学] R378.61[医药卫生—基础医学]