4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布  

Establishment of Markers for Four Blast Genes and Marker Distribution in Rice Parents

作　　者：高利军[1,2] 高汉亮[1] 颜群[1] 周萌[1] 周维永[1] 张晋[1] 邓国富[1,2] 

机构地区：[1]广西农业科学院,广西南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,广西南宁530007

出　　处：《杂交水稻》2010年第S1期294-298,共5页Hybrid Rice

基　　金：国家"863"计划子课题(2006AA100101-2);广西科技创新能力与条件建设项目(桂科能0815011-1-1-9);广西科技基础条件平台建设项目(09-007-08)

摘　　要：建立稻瘟病抗性基因的分子标记对于培育抗稻瘟病水稻品种有重要意义。本文利用抗稻瘟病基因Pi9、Pi2、Pi5和Pita基因序列与日本晴等位基因的序列差异,建立4个基因的共显性分子标记M-Pi9、M-Pi2、M-Pi5和M-Pita,并用这4个分子标记检测24个抗稻瘟病单基因系和48份水稻亲本,结合48份水稻亲本对广西采集的稻瘟病菌ZB1和ZB13苗瘟抗性鉴定结果,验证4个分子标记的特异性和有效性。结果表明,M-Pi9仅在含Pi9和Piz的单基因系中检测出特异带;M-Pi2仅在含Pi2和Pizt的单基因系中检测出特异带;M-Pi5仅在含Pi5、Pi3和Pii的单基因系中检测出特异条带;M-Pita在含Pita和Pita2的单基因系中检测出特异带。具有M-Pi2特异条带的水稻亲本对ZB1和ZB13均表现出抗性,而具有M-Pi9、M-Pi5或M-Pita特异条带的水稻亲本对ZB13均表现出抗性。The establishment of molecular markers of rice blast resistance genes was a powerful strategy in rice blast resistance breeding.In present study,PCR markers for four blast resistance genes(Pi9、Pi2、Pi5 and Pita) were screened by the primers designed based on the sequences of four blast genes and their variances in Nipponbare.These markers were tested in 24 rice lines carrying a single blast resistance gene and 48 rice parents.Rice blast resistances of 48 rice parents were also tested with rice blast pathogens ZB1 and ZB13 collected from Guangxi.The results showed that these markers were effective to identify blast resistance genes.The marker M-Pi9 showed a specific amplified band in the lines carrying the gene Pi9 or Piz among the 24 rice lines with a single blast resistance gene;M-Pi2 showed specificity in the lines with gene Pi2 or Pizt;M-Pi5 showed specificity in three lines with genes Pii,Pi3 or Pi5;and M-Pita showed specificity in the lines with the gene Pita or Pita2.Furthermore,we found that the rice parents with the specific marker M-Pi2 showed resistance to rice blast pathogen ZB1,while that with four markers(M-Pi9,M-Pi2,M-Pi5 and M-Pita) showed resistance to rice blast pathogen ZB13.

关 键 词：水稻 分子标记 稻瘟病 抗性基因 

分 类 号：S511[农业科学—作物学]