叶绿体遗传转化技术的研究进展及应用于杂交水稻机械化制种的初步探索  

Advances in Chloroplast Genetic Transformation and a Preliminary Exploration on Its Use in Mechanized Hybrid Rice Seed Production

作　　者：李丁[1] 谢灵灵 崔玲玲[2] 严礼 左佳 贺荣华 赵迎曦 曹孟良[3] 

机构地区：[1]中南大学研究生院隆平分院,湖南长沙410125 [2]湖南隆平高科基因科技有限公司,湖南长沙410125 [3]国家杂交水稻工程技术研究中心,湖南长沙410125

出　　处：《杂交水稻》2010年第S1期332-337,共6页Hybrid Rice

基　　金："863"重点项目(2006AA100101;2009AA101100);"973"项目(2007CB109007);国家科技支撑计划(2007BAD77B00);国家转基因重大专项(2008ZX08001;2009ZX08001-010B)

摘　　要：叶绿体转化是继核转化之后在基因工程研究中出现的新热点。叶绿体转化具有表达高、可同时进行多基因转化、无基因沉默现象等许多优点。概述了植物叶绿体基因组特点、遗传转化技术原理以及最近研究进展,并对其应用于杂交水稻机械化制种做了初步探索。Chloroplast is a new hotspot in the field of plant transformation system of plant genetic engineering.Plastid transformation has several advantages:high expression,multiple expressed genes in a single transformation event,absence of gene silencing,et al.In this paper,chloroplast genome characteristics,principle of transformation technology and recent advances were reviecoed.Moreover,a preliminary exploration on the application of chloroplast genetic transformation in mechanized hybrid rice seed production was conducted.

关 键 词：杂交水稻 叶绿体转化 机械化制种 

分 类 号：S233.71[农业科学—农业机械化工程]