免疫组织化学法检测北京地区牛肝脏中戊型肝炎病毒抗原  

Detection of the Hepatitis E Virus (HEV) Antigen by Immunohistochemistry in Cattle Liver

作　　者：尹君[1] 佘锐萍[1] 李睿文[1] 孙泉[1] 毛晶晶[1] 于品[1] 吕东强[1] 潘亚涛[1] 

机构地区：[1]中国农业大学动物医学院,北京100094

出　　处：《动物医学进展》2010年第S1期55-58,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30871853)

摘　　要：收集北京地区2月龄犊牛49份肝脏样品,34份血清样品,屠宰肉牛肝脏样品153例和60份屠宰牛血清样品。肝脏样品经戊二醛-多聚甲醛固定,包埋,切片,最后进行HE及免疫组化染色。肝脏HE染色结果显示,大多数的肝脏中均出现炎性细胞浸润及纤维组织增生。免疫组织化学染色结果显示,在49例犊牛肝脏样品中,阳性检出率为48.8%(24/49),而153例屠宰牛肝脏样品阳性检出率仅为13.7%(20/153)。血清样品HEV ELASA检测结果表明,34份犊牛血清中检测出1例阳性样品,阳性率为2.95%,60份屠宰牛血清样品检出阳性样品2例,占3.33%。49 liver samples and 34 serum samples from two months old calves,153 liver samples and 60 serum samples from slaughtered cattles were collected for this study.Embed by glutaraldehyde-paraformaldehyde and then stained by HE and immunohistochemistry,the result of the HE staining shows that the majority of the liver samples has suffered from inflammatory cell infiltration and a tendency of liver fibrosis.The results also show that the positive rates detected by immunohistochemical staining in the 49 cases of bovine liver samples were as high as 48.8%(24/49),while samples of the 153 cases positive rate was merely 13.7%(20/153).All the positive signal detected among the liver sample cases are distributed irregularly.one cases of calf(2.95%)and two cases(3.33%)of 60 slaughtered cattles serum samples were detected by using swine hepatitis E ELASA.

关 键 词：戊型肝炎病毒 免疫组化 牛肝脏 

分 类 号：S858.23[农业科学—临床兽医学]