犬肝脏中戊型肝炎病毒抗原的检测及组织病理学观察  

Detection of the Hepatitis E Virus (HEV) Antigen and Histopathologic Observation in Liver of Pet Dog

作　　者：李睿文[1,2] 周寅[1] 佘锐萍[1] 李丰昌 尹君[1] 毛晶晶[1] 孙泉[1] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]河北农业大学中兽医学院,河北定州073000 [3]北京永昌动物医院,北京100000

出　　处：《动物医学进展》2010年第S1期58-62,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助项目(项目编号:30871853)

摘　　要：为探讨宠物犬戊型肝炎病毒(HEV)的感染情况,试验采集了21例宠物犬肝脏,采用免疫组织化学染色方法对上述肝脏组织中的HEV-Ag进行了检测,同时应用H.E染色和Mallory三染色法对其病理形态学变化进行了观察。结果表明在21例宠物犬肝脏中均检出了HEV抗原。组织病理学观察可见肝脏有明显的炎症变化。提示宠物犬感染HEV程度很高,并且是人感染HEV潜在传染源,具有重要的公共卫生意义。In order to invest the prevalence of HEV in pet dogs,21 livers of dog were collected to detect the hepatitis E virus(HEV) antigen by immunohistochemical method,and observe pathological changes of dog's liver by HE staining and Mallory's staining.The results show that 21 livers of pet dog may be detected HEV-Ag,and positive rate was 100%;and the histopathologic changes of the livers of pet dog consistent with the pathologic changes of liver of human that infected HEV.It can be seen that the prevalence of HEV is high degree in pet dogs.The pet dogs infected HEV Is a potential source to infect human,and it is isignificance mt in public health.

关 键 词：宠物犬 戊型肝炎 免疫组织化学 组织病理学 

分 类 号：S858.292[农业科学—临床兽医学]