猪源新城疫病毒JL01株分离鉴定及F基因遗传进化分析  

Isolation and identification of swine NDV JL01 strain and phylogenetic analysis of F gene

作　　者：鲁会军[1] 金扩世[1,2] 韩松[1] 金宁一[1,2] 

机构地区：[1]中国人民解放军军事医学科学院军事兽医研究所,吉林长春130062 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《动物医学进展》2010年第S1期103-107,共5页Progress In Veterinary Medicine

基　　金：国家"973"计划资助项目(2005CB23005);自然科学基金项目(30800828)

摘　　要：2000年,在吉林省某猪场发生具有较高发病率和死亡率的急性传染病,发病率达到40%～50%,病死率15%,取其脾、肺、肾等组织进行电镜观察,可见副黏病毒样颗粒,表明其病原可能为某种猪源副黏病毒,命名为JL01株。在对该病毒的的血凝、血凝抑制等生物学特性进行鉴定后,初步确定该种猪副黏病毒为猪源新城疫病毒。病毒回归试验表明,纯化的病毒对猪仍有较强的致死性,并可从死亡猪体内分离到新城疫病毒。该病毒对鸡胚平均致死时间(MDT)、脑内致病指数(ICPI)和半数致死量(EID50)分别为55.2 h、1.60和10-7.5/0.1mL,表明该毒株属于新城疫病毒强毒株。在此基础上,采用RT-PCR方法克隆了猪源新城疫病毒F基因,与其它10株NDV毒株进行序列比较分析,结果表明,JL01株与B1、LaSota、Clone30等经典的新城疫病毒弱毒株同源性较高(91.5%～98.5%),与ZJ1、Mukteswar等强毒株的同源性较低。F基因氨基酸裂解位点的序列为112G-K-Q-G-R-L117,与弱毒株序列完全相同。基因分型结果表明,JL01株属于基因Ⅰ型。因此,本研究所分离到感染猪的新城疫病毒属于基因变异的新城疫病毒弱毒株,但其致病力与强毒株相当。An outbreak of fever and dyspnea with high incidence rate and case fatality rate occurred among pigs in a pigfarm in Jilin province,China,in 2000.The paramyxovirus-like particles could be observed in lungs,spleens and kidneys of the dead pigs under the transmission electron microscope.A Newcastle disease virus(NDV) isolate designated as JL01 was determined as the causal agent for the disease outbreak.The purified virus was reinoculated into pigs,and then the pigs infected with the virus showed similar signs,and the HI antibody of NDV could be detected from the reinoculated pigs.The MDT,ICPI and EID50 of the JL01 isolate was 55.2h,1.60 and 10-7.5/0.1 mL respectively.The F gene of JL01 was cloned and sequenced,and the results showed that the identities of F gene shared by JL01 and NDV avirulent reference strains were from 91.5% to 98.5%,and could be ascribed to NDV genotype Ⅰ.Thus,the swine NDV JL01 strain should be a avirulent strain with genovariation,but the virulence of JL01 is just same as velogenic strains.

关 键 词：猪源新城疫病毒 分离 鉴定 F基因 分析 

分 类 号：S852.659.5[农业科学—基础兽医学]