奶牛隐性乳房炎病原菌的分离及药敏试验  

Isolation,Identification and Medicine Sensitivity Test of Pathogenic Bacteria of Dairy Cow Mastitis

作　　者：付新城[1] 庞树坤 

机构地区：[1]河北省廊坊市农业局动物卫生监督所,河北廊坊065000 [2]河北省涿州市动检站,河北涿州072750

出　　处：《动物医学进展》2010年第S1期169-174,共6页Progress In Veterinary Medicine

摘　　要：选择某牛场50头临床健康奶牛,采用CMT法进行隐性乳房炎检测,其中28头奶牛患有隐性乳房炎,发病率为56%;在被检的200个乳样中患有隐性乳房炎的乳区84个,乳区阳性率为42%。对阳性乳样进行细菌分离鉴定,从28头患有隐性乳房炎的奶牛的84个乳样中检出13种共98个菌株,其中葡萄球菌44个,占44.90%;金黄色葡萄球菌32个,占32.65%;链球菌20个,占20.40%;大肠埃希菌11个,占11.22%。由葡萄球菌、链球菌和大肠埃希菌引起的乳房炎占整个比例的75.51%。表明引起奶牛隐性乳房炎的病原菌以葡萄球菌、链球菌和大肠埃希菌为主,而且单纯由某一种病原菌引起的病例较少,多数是由2～3种甚至是3种以上的病原菌引起,混合感染率为83.33%。药敏试验结果表明,临床使用较多的青霉素和链霉素对奶牛乳房炎的主要病原菌有较强的耐药性,而对庆大霉素、丁胺卡那霉素、环丙沙星和林可霉素等药物则较为敏感。In a certain herd(50),the investigation showed that of subclinical mastitis is 56%(28 of 50).The GVFBX quarter infection rate of subclinicate is 42%(84 of 200).Ninety-eight strains bacteria were isolated from eighty four milk samples of twenty eighty dairy cows mastitis in some areas.Results showed that 44 strains of them were Staphylococci(44.90%),20 were Streptococci(20.40%),11 were Escherichia coli(11.22%).The cases of cow mastitis caused with Staphylococci and Escherichia coli were 75.51%,which indicated that the Staphylococcus aureus was the main pathogenic bacteria,and 83.33% were mixed with two-three or more than three types of bacteria.In addition,the medicine sensitivity test presented that those pathogenic bacteria were resistant to Penicillin and Streptomycin frequently used,while Gentamicin,Amikacin,Ciprofloxacin and Lincomycin had fairly inhibiting effect on the pathogenic bacteria.

关 键 词：奶牛乳房炎 病原菌 分离鉴定 药敏试验 

分 类 号：S858.23[农业科学—临床兽医学]