异养硝化-好氧反硝化菌WXZ-8的脱氮产物N_2O和N_2研究  

Experimental Study on N_2O and N_2——Products of Ammonium Removal by Heterotrophic Nitrification-Aerobic De-nitrification Strain WXZ-8

作　　者：尹明锐[1] 汪苹[1] 廖小红[1] 张正洁[1] 马洁峰[1] 于群[1] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100048

出　　处：《环境科学与技术》2010年第8期16-19,23,共5页Environmental Science & Technology

基　　金：国家"十一五"科技支撑计划课题(2007BAK36B07);国家高技术产业发展项目"味精行业高浓度废水处理关键技术高技术产业化示范工程"(内发改高技字[2008]163号)

摘　　要：研究了异养硝化-好氧反硝化菌WXZ-8(Bacillus cereus)的异养硝化性能,并采用GC-ECD(Gas Chromatography-Electron Capture Detector)、GC-TCD(Gas Chromatography-Thermal Conductivity Detector)方法分别测定了脱氮过程中的气体产物N2O和N2。为了改进密封反应的溶氧条件,采用纯氧密封摇瓶培养和空气密封摇瓶培养两个对照条件下进行培养。结果表明:(1)空气密封瓶实验条件下,WXZ-8产生的N2O为0.00945mg,N2为9.5005mg,分别占从水体中脱除的氮的0.047%和47.09%,另外,同化合成的氮含量占48.74%;(2)纯氧密封瓶实验条件下,WXZ-8产生的N2O为0.00463mg,N2为9.686mg,分别占从水体中脱除的氮的0.024%与49.57%,同样,同化合成的氮含量占49.23%。两组对照实验表明,WXZ-8菌是一株高效、产生低水平量N2O的性能良好的异养硝化-好氧反硝化菌,且纯氧培养条件更有利于控制N2O的逸出。The aerobic de-nitrification strain WXZ-8,belonging to Bacillus cereus,was cultured and isolated in the lab,and then was studied with respect to its heterotrophic nitrification function by measuring the gaseous products (N2O and N2) using GC-ECD and GC-TCD.A comparative study was carried out in order to investigate whether the air within the air-proof flask could provide enough O2 to sustain the whole nitrification process,i.e,one flask was filled with air and the other flask with pure oxygen.The finding of the experimental study showed that Strain WXZ-8 was an effective heterotrophic nitrification bacterial strain for removing ammonium from water with limited emission of N2O;and pure O2 cultural condition was better in controlling emission of N2O.

关 键 词：异养硝化 脱氮 N2O控逸 N2 

分 类 号：X703[环境科学与工程—环境工程]