微颗粒饲料中添加谷氨酰胺对大黄鱼稚鱼生长、存活及消化酶活力的影响  

Effects of Dietary Glutamine on Growth,Survival and Activities of Selected Digested Enzymes of Large Yellow Croaker (Pseudosciaena crocea) Larvae

作　　者：高进[1,2] 艾庆辉[1,2] 麦康森[1,2] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室 [2]中国海洋大学水产学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2010年第S1期49-54,共6页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30400335)资助

摘　　要：以初始体质量为(2.75±0.31)mg的大黄鱼(Pseudosciaena crocea)稚鱼(15日龄)为实验对象,在基础饲料中分别添加0.00%(对照组)、0.60%、1.20%和1.80%的谷氨酰胺,并用甘氨酸调节总蛋白质水平一致,制作而成4种粗蛋白58%左右、粗脂肪16%左右的实验微颗粒饲料,在室内养殖系统中投喂大黄鱼稚鱼30 d,研究饲料谷氨酰胺对大黄鱼稚鱼生长、存活及消化酶活力的影响。实验结果表明,大黄鱼稚鱼的特定生长率和存活率随饲料中谷氨酰胺含量的升高有上升的趋势,但差异未达到显著水平(P>0.05)。饲料中谷氨酰胺的添加未对大黄鱼稚鱼肠段、胰段胰蛋白酶活力和淀粉酶活力,肠段氨基肽酶活力和碱性磷酸酶活力产生显著的影响(P>0.05)。饲料中添加谷氨酰胺未能对大黄鱼稚鱼的生长、存活和消化酶活力产生显著的影响,可能与基础饲料中鱼粉、鱼肉水解蛋白等蛋白源所含有的谷氨酰胺已经达到或超过稚鱼吸收、利用谷氨酰胺的阈值,额外添加的谷氨酰胺不能被吸收利用而未能表现出促进效果。本实验条件下,大黄鱼稚鱼基础饲料中无需额外添加谷氨酰胺即可满足稚鱼正常生长发育的需要。Four experimental micro-diets supplemented with 0.00%(control),0.60%,1.20% and 1.80% glutamine were fed to large yellow croaker larvae(15days post hatch,initial body weight 2.75±0.31 mg) for 30 days to investigate the effects of glutamine on growth,survival and activities of selected digestive enzyme of larvae.Glycine was used to adjust the level of crude protein to be about 58%,and crude lipid were about 16% in the diets.The results showed that specific growth rate and survival of large yellow croaker larvae increased with the supplementation of glutamine,but no significant difference was observed among dietary treatments(P>0.05).There were no significant differences in activities of amylase and trypsin in intestinal or pancreatic segments,alkaline phosphatase and aminopeptidase N in intestinal segments among fish fed different diets(P>0.05).In this experiment,dietary glutamine didn't show significant influence on growth,survival and activities of digestive enzyme of large yellow croaker larvae.This may be related to the content of glutamine contained in fish meal,hydrolysated fish meal and other protein sources in the diets,which perhaps had satisfied or exceeded the limit that the larvae could absorb and utilize.So additional glutamine supplementation could not be absorbed and utilized to perform the effects.The results showed that the basal diet could satisfy the nutrition requirement of the larvae,and no more additional glutamine was required by large yellow croaker larvae.

关 键 词：大黄鱼稚鱼 谷氨酰胺 生长 存活 消化酶活力 

分 类 号：S963[农业科学—水产养殖]