活的非可培养状态溶藻胶弧菌复苏后的致病性及基因表达  

Virulence Retention and Gene Expression of Vibrio alginolyticus VIB 283 Resuscitated from Viable but Nonculturable State

作　　者：姜荧安[1] 陈吉祥[1] 贾俊涛[2] 郭倩茹[3] 赵明君[1] 

机构地区：[1]中国海洋大学,山东青岛266003 [2]山东出入境检验检疫局,山东青岛266002 [3]广东海洋大学,广东湛江524088

出　　处：《中国海洋大学学报（自然科学版）》2010年第S1期142-146,共5页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30972275);农业部公益性行业专项项目(nyhyzx07-046);国家质检总局科研项目(2010IK180)资助

摘　　要：溶藻胶弧菌广泛分布于海洋环境,是海洋生物及人类的条件性致病菌。溶藻胶弧菌在不良环境进入活的非可培养状态,当条件适宜时复苏为可培养形式。本文研究了溶藻胶弧菌由活的非可培养状态复苏为可培养状态时的生理特征及对斑马鱼的致病性,结果发现复苏为可培养状态的溶藻胶弧菌对紫外辐射、热激、冷激的抵抗力没有明显改变。用RT-PCR未检测到VBNC状态溶藻胶弧菌tox S和tox R基因的表达,而复苏后的细胞检测到这些基因的表达。复苏后的溶藻胶弧菌对斑马鱼的LD50为6.25×106CFU/尾,与野生菌株(LD50为4.80×106CFU/尾)没有显著差别。Vibrio alginolyticus is widely distributed in marine environment.It is an opportunistic pathogen of human being and marine animals.The bacterium is able to adapt to environmental stresses and enter into the viable but nonculturable(VBNC) state.The VBNC cells could be recovered to culturable state under certain conditions.This study investigated the physiological characteristics and pathogenicity to zebra fish of the resuscitative cells.The sensitivity of the resuscitative cells to UV,high temperature(42℃) and cold stresses did not change obviously when compared with that of the normal cells.Reverse transcription-polymerase chain reaction(RT-PCR) was used to identify expression level of virulence related toxS and toxR genes of the resuscitative cells.The expressions of toxS and toxR genes were not detected in the VBNC cells but in the resuscitative cells.The resuscitative cells retained their virulence when they were intraperitoneally inoculated into zebra fish.The LD50 of the wild-type cells and the resuscitative cells was 4.80×106CFU per fish and 6.25×106CFU per fish respectively,which showed no significant difference.

关 键 词：溶藻胶弧菌 活的非可培养状态 复苏 致病性 基因表达 

分 类 号：S941[农业科学—水产养殖]