Cloning and Expression Level Analysis of Melanocyte-stimulating Hormone Receptor 1 Gene(MC1R) in Alpacas with Different Coat Color  

作　　者：REN Yu-hong REN Bin FAN Rui-wen ZHU Zhi-wei YANG Yong LI Hui DONG Chang-sheng 

机构地区：[1]College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China [2]College of Animal Science and Veterinary Medicine,Agricultural University of Hebei,Baoding 071000,China

出　　处：《畜牧兽医学报》2010年第S1期21-25,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：supported by the National Natural Science Foundation of China(No.30501070);Shanxi Natural Science Foundation(No.20041099);President Foundation of Agricultural University of Hebei (BS2007023)

摘　　要：Specific primers for the MC1R gene of alpacas(GenBank EU1358800) were designed to amplify the cDNA sequence using RT-PCR to seek variation in the sequence and explore the relationship between the expression level of MC1R gene and alpaca coat color.The MC1R gene from white alpaca was cloned successfully and sequence analysis verified that the MC1R gene,encoding 317 amino acids,was 1081 bp in length.Compared with the existing sequence in GenBank,sequence identity was 99.9%and 7 mutations were found.Primers,designed from the sequence obtained,were used to assess the relative expression of MC1R in alpacas of different coat color using QRT-PCR and SPSS 13.0 software.Relative expression of MC1R in the skin of brown alpacas was 4.32 times higher than that in white alpacas after normalization with GAPDH(P【0.01),indicating that MC1R expression may be related to coat color of alpacas.Specific primers for the MC1R gene of alpacas(GenBank EU1358800) were designed to amplify the cDNA sequence using RT-PCR to seek variation in the sequence and explore the relationship between the expression level of MC1R gene and alpaca coat color.The MC1R gene from white alpaca was cloned successfully and sequence analysis verified that the MC1R gene,encoding 317 amino acids,was 1081 bp in length.Compared with the existing sequence in GenBank,sequence identity was 99.9%and 7 mutations were found.Primers,designed from the sequence obtained,were used to assess the relative expression of MC1R in alpacas of different coat color using QRT-PCR and SPSS 13.0 software.Relative expression of MC1R in the skin of brown alpacas was 4.32 times higher than that in white alpacas after normalization with GAPDH(P<0.01),indicating that MC1R expression may be related to coat color of alpacas.

关 键 词：ALPACA melanocyte-stimulating hormone receptor 1 gene(MC1R) cloning QRT-PCR gene expression level 

分 类 号：S829.9[农业科学—畜牧学]