武汉东湖浮游生物宏基因组与环境重金属的关系  

Relationships between metagenome of plankton community and heavy metals in Lake Donghu,Wuhan

作　　者：颜庆云[1] 余育和[1] 冯伟松[1] 

机构地区：[1]中国科学院水生生物研究所水生生物多样性与保护重点实验室,湖北武汉430072

出　　处：《中国环境科学》2010年第S1期52-56,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(30770298);淡水生态与生物技术国家重点实验室开放课题(2008FB016)

摘　　要：采用针对16SrRNA和18SrRNA基因的引物,对武汉东湖不同营养水平湖区的浮游生物宏基因组进行凝胶电泳分析(PCR-DGGE)的指纹分析,并探讨宏基因组与环境重金属的关系.结果表明,PCR-DGGE分析共获得82条谱带,其多态率为82.9%,特有谱带占31.7%.I^V站点的谱带分别为36,34,44,50,56条.5个站点浮游生物群落PCR-DGGE指纹结构的聚类分析(UPGMA)分成两大类:I、II、Ⅲ站为一类,IV、V站为一类,这与重金属(铅、铬、汞、镉、砷、铜)浓度对各站点的聚类分枝关系相一致.相关分析显示,变性梯度凝胶电泳(DGGE)谱带所代表的分类单元数(OTUs)与镉、铬的浓度存在较明显的相关性,其相关系数分别为-0.942(P<0.05)和-0.928(P<0.05).Metagenome of plankton community in Lake Donghu was investigated by PCR-DGGE fingerprinting of the 16S rRNA and 18S rRNA genes, respectively. Then the relationships between the DGGE fingerprints of the plankton community and heavy metals were explored. A total of 82 DGGE bands were detected, of which 82.9% were polymorphic bands and 31.7% were unique bands. The bands detected at station I～V were 36,34,44,50 and 56, respectively. The five investigated stations were classified into two groups using UPGMA clustering based on the DGGE fingerprints of plankton community. Station I and II were found to initially group together into one cluster, which was later joined by station III. Station IV and V made up the second group. These group divisions were consistent with the UPGMA clustering based on the concentrations of the heavy metals (Pb, Cr, Hg, Cd, As, Cu). The correlation analysis indicated that the operational taxonomic units (OTUs, one band-one OTU in principle) were correlated with the heavy metals of Cd (R= -0.942, P < 0.05) and Cr (R = -0.928, P < 0.05).

关 键 词：浮游生物群落 宏基因组 变性梯度凝胶电泳(DGGE) 重金属 

分 类 号：X524[环境科学与工程—环境工程]