不同获能液对猪精子获能效果的初步观察  

Initial Comparison of CTC Staining among Different Porcine Sperm Capacitation Impacts

作　　者：赵曌[1] 徐捷[1] 赵菊[1] 张志刚[1] 高建明[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《北京农学院学报》2009年第1期33-35,共3页Journal of Beijing University of Agriculture

基　　金：北京市教委资助项目(KM200710020014)

摘　　要：为了探讨不同获能液对猪精子获能效果的影响,以新鲜猪精液为材料,进行mTBM+咖啡因、mTBM+咖啡因+BSA,mTBM+肝素三种获能液获能效果的比较。利用金霉素荧光染色法对其获能效果进行检验。结果表明,mTBM+肝素组诱发B型(获能)精子率极显著高于mTBM+咖啡因组(P<0.01),显著高于mTBM+咖啡因+BSA组(P<0.05);三种获能液诱发的AR型(顶体反应)精子率差异不显著(P>0.05)。说明,添加肝素更有助于提高猪精子获能效果。In this experiment, the discrepancy was compared among mTBM＋ caffenine, mTBM＋ caffenine ＋ BSA, mTBM q-heparinize for porcine sperm capacitation in vitro with chlortetracycline fluorescence （CTC） staining. The results showed that the rates of sperm capacitation by mTBM＋heparinize was significant higher than mTBM＋caffenine（P〈0.01） and mTBM＋caffenine＋BSA（P〈0.05） ; there on the rates of acrosome reaction among groups there（P〉0. 05）. It suggested that mTBM＋heparinize was suitable for the application of in vitro sperm capacitation of porcine.

关 键 词：猪 精子获能 金霉素荧光染色 

分 类 号：S828.534[农业科学—畜牧学]