秋水仙素对大蒜根尖细胞有丝分裂的影响  

Effect of Colchicine on the Mitotic Division of Root Tip Cells of Allium sativum

作　　者：赵东利[1] 周清波[1] 蔡娜[1] 郭伟[1] 任欣[1] 

机构地区：[1]大连大学医学院,辽宁大连116622

出　　处：《北京农学院学报》2009年第1期79-80,共2页Journal of Beijing University of Agriculture

基　　金：大连大学本科生创新基金项目

摘　　要：用0.1%和0.01%浓度的秋水仙素溶液分别处理大蒜根尖6,12,24,48,72,96h,大蒜根尖细胞有丝分裂指数呈现上升趋势,以0.01%的秋水仙素处理大蒜根尖24h达到最高值14.29%,是对照的6.8倍,之后随处理时间延长,有丝分裂指数下降。0.1%浓度组数值一直低于0.01%浓度组。结果表明秋水仙素在一定浓度和一定时间范围内可以促进大蒜根尖细胞有丝分裂,而超过一定浓度或处理时间过长,将会抑制有丝分裂。Effects of colchicine on the mitotic division of root tip cells of Alliurn sativum were studied in this paper. Roots of Alliurn sativum were treated respectively for 6,12,24,48,72,96 h with two concentrations of colchicine 0.1% and 0. 01%. The results indicated that the mitotic index firstly rose with the increase of treating time of colchicine, and the highest mitotic index（14. 29%） could be gained, which was 6.8 times higher than the controlled group after the root tip cells of Allium sativum were treated for 24 hour by 0.01% colchicines. And the mitosis index began to decrease when the root tip cells were treated for a longer time. All data from 0.01% colchicine were higher than those from 0. 1% colchicine. It suggested that the mitosis of Allium sativum root tip cells was enhanced in appropriate concentrations of colchicines and treating time, but the improper concentration of colchicine and duration time could result in the contrast.

关 键 词：秋水仙素 大蒜 根尖 有丝分裂指数 

分 类 号：S633.401[农业科学—蔬菜学]