MMP-2在缺氧缺血／复氧再灌注损伤新生大鼠星形胶质细胞表达及FDP干预研究  

Expression of MMP-2 by cultured astrocytes and effects of FDP intervention on hypoxia-ischemia/reoxygenation injury in neonatal rats

作　　者：陈蓉[1] 姚裕家[2] 王予川[1] 

机构地区：[1]贵州省人民医院儿科,贵州贵阳550002 [2]四川大学华西第二医院新生儿科,四川成都610041

出　　处：《临床儿科杂志》2009年第4期363-367,共5页Journal of Clinical Pediatrics

基　　金：教育部博士点基金资助(No.20050610071)

摘　　要：目的研究新生大鼠星形胶质细胞(AC)基质金属蛋白酶2(MMP-2)在缺氧缺血/复氧再灌注(复氧)损伤中的变化和1,6-二磷酸果糖(FDP)对AC缺氧/复氧损伤MMP-2表达的影响,为缺氧缺血性脑损伤的机制和治疗提供线索。方法采用免疫激光共聚焦的方法检测体外培养的AC在缺氧/复氧不同时段和给予FDP干预时MMP-2的表达。结果随着缺氧/复氧时间延长,MMP-2蛋白在AC的表达明显增高;FDP保护组及治疗组较相应时段缺氧复氧组MMP-2蛋白表达均明显降低。且随着缺氧/复氧时间延长,AC形态变化明显,主要表现为细胞间连接减少,足突减少;FDP保护12 h组及FDP治疗6 h组,AC形态变化明显减轻。结论在缺氧/复氧损伤时,AC的MMP-2表达增高,AC间的相互连接破坏,影响AC正常形态、功能的维持。FDP可通过降低MMP-2的表达,以维持AC间相互连接和AC的正常形态,减轻缺氧/复氧对AC的损伤。Objective To study the expression of matrix metalloproteinase-2 （MMP-2） by cultured astrocytes （AC） of neonatal rats during hypoxia-ischemia/reoxygenation, and to explore the effect of fructose-1, 6-disphosphate （FDP） on MMP-2 expression in AC of neonatal rats with hypoxia-ischemia/rcoxygenation （HI/RO） injure, for providing laboratory evidence on mechanism and clinical management of hypoxia-ischemia brain damage. Methods The original cultivating mature purified AC were randomly divided into five groups. In HI group, the astrocytes were exposed to 1% O2 and cultured without FBS and glucose medium for 12 h and 24 h respectively; In FDP protected group, the astroeytes were exposed to 1% O2, cultured without FBS and glucose medium added with FDP 20 μ/ml into the medium at 12 h and 24 h respectively; In RO group, the astroeytes were subjected to 12 h HI and then transferred to ordinary cultured medium at 6 h, 12 h and 24 h respectively; In FDP treated group, the astrocytes were subjected to 12 h HI, then transferred to ordinary cultured medium and added with FDP 20 μ/ml into the medium at 6 h, 12 h and 24 h respectively; Control group was normal oxygen cultivated group. The expressions of MMP-2 protein were determined by immunofluorescence. Results As the time of HI/RO prolonged, the expression of MMP-2 protein by AC significantly increased （P 〈 0.05）. Compared with the same period of HI group, expression of MMP-2 protein by AC significantly decreased in FDP protected group （P 〈 0.05）. Compared with the same period of RO group, expression of MMP-2 protein by AC significantly decreased in FDP treated group （P 〈 0.05）. As the time of HI/RO prolonged, the morphological character of AC obviously changed and the changes mainly were decrease of the contacts among cells and lessening of cell foot process. The morphological character of AC in FDP protected group at 12 h and FDP treated group at 6 h were only slightly changed. Conclusions During the HI/RO injure, the expression of MMP-2 by

关 键 词：星形胶质细胞 基质金属蛋白酶2 缺氧缺血 复氧 1 6-二磷酸果糖 

分 类 号：R722.1[医药卫生—儿科]