甲型肝炎病毒Vero细胞适应株(YN5株)的传代稳定性  

The stability of vero cell adapted HAV strain (YN5) during passage cultivation

作　　者：马波[1,2] 吴克 谭顺革 代云波 施松明 魏云林[1] 

机构地区：[1]昆明理工大学化学工程学院,云南昆明650224 [2]云南沃森生物技术有限公司,云南昆明650106 [3]上海惠生生物工程有限公司,上海201203

出　　处：《云南大学学报（自然科学版）》2007年第S3期335-337,共3页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家高技术研究发展专项经费资助项目(2002AA223313);云南省科技计划资助项目(2001XY01)

摘　　要：为检验甲型肝炎病毒Vero细胞适应株(YN5株)的传代稳定性,采用连续传代的方法,进行24 d适应性培养,结果表明随着增殖代次的增加,滴度呈递增趋势,18代后滴度趋于稳定.至23代时感染性滴度达8.67LogCCID_(50)/mL,抗原滴度1:4 096.25代毒种经连续3批中试规模生产,其抗原增殖水平稳定,所制备的灭活疫苗免疫原性良好.证明HAV YN5株已适应Vero细胞,增殖稳定,为大规模生产提供了有效保障.To investigate the stability of HAV adapted strain( YN5)of Vero cell in pilot scale, serial passage was used to carry out 24 d adaptability cultivation. It showed that the titre increased progressively and tended to stable after 18 th generation. The infective titre reached 8.67 LogCCID_(50)/mL and antigen titre reached 1:4 096 at 23 rd generation. The immunogenicity of the deactivated vaccine precipitated by the virus strain of 25 th generation is fine, while antigen proliferation level was stable after continuous 3 batches of pilot scale production.It demonstrated that HAV YN5 , whose conservation and proliferation were stable, had already adapted to Vero cell ,and it ensured the large scale manufacture effectively in future.

关 键 词：HAV YN5株 VERO细胞 稳定性 

分 类 号：R373[医药卫生—病原生物学]