碳纳米管和纳米氧化锌修饰的尿酸传感器的研制  

Study on the uric acid biosensor modified with nanomaterial

作　　者：卢旭晓[1] 杜霞[1] 薛玲[1] 白慧萍[1] 尹晓燕[1] 李宝柱[1] 张亚平[2] 杨云慧[1] 

机构地区：[1]云南师范大学化学化工学院,云南昆明650092 [2]昆明师范高等专科学校,云南昆明650031

出　　处：《云南大学学报（自然科学版）》2007年第S1期216-221,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省自然科学基金资助项目(2006B0028M);云南师范大学博士科研启动经费资助项目

摘　　要：利用碳纳米管和纳米氧化锌对尿酸的电催化氧化作用,研制了一种新型的基于尿酸氧化酶的生物传感器.考察了pH、电位等条件对传感器测定尿酸的影响.结果表明在pH 6.98,电位为0.61 V条件下传感器检测尿酸的线性范围为3.38×10-6~1.59×10-3mol/L,检出下限为1.48×10-6mol/L,该传感器稳定性好,制作简单.可用于实际尿样中尿酸的测定,为尿酸的测定提供了一种新的手段.A uric acid biosensor is constructed by using nano ZnO,carbon nanotube and chitosan as immobilization matrix immobilize uricase on aglassy carbon.The effect of pH and applied potential were investigated.The result shows that when the pH is 6.98,and the potential is 0.61 V,the currents increase linearly with the concentration of uric acid in the range of 3.38×10-6—1.59×10-3 mol/L.The calculated detection limit is 1.48×10-6 mol/L.It is successfully used in the detection of uric acid level in human urine with high stability,sensitivity and anti-poisoning ability.

关 键 词：尿酸 尿酸酶 生物传感器 碳纳米管 纳米氧化锌 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]