Developmental expression of amphioxus RACK1  

作　　者：HUANG XiangWei1, ZHANG Wei1, LI XinYi1, ZHANG XiaoHui1, LI BaoJun1, MAO BingYu2 & ZHANG HongWei1 1 Institute of Developmental Biology, College of Life Science The Key Lab of Experimental Teratology, Ministry of Education, Shandong University, Jinan 250100, China 2 Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 

出　　处：《Science China(Life Sciences)》2007年第3期329-334,共6页中国科学（生命科学英文版）

基　　金：the National Natural Science Foundation of China (Grant Nos. 30270693 and 30570967)

摘　　要：Vertebrate RACK1 plays a key role in embryonic development. This paper described the cloning, phy- logenetic analysis and developmental expression of AmphiRACK1, the RACK1 homologous gene in amphioxus. Phylogenetic analysis indicated that amphioxus RACK1 was located at the base of verte- brate clade. AmphiRACK1 expression in lithium-treated embryos was also examined. During embryonic development, AmphiRACK1 was expressed strongly in cerebral vesicles, neural tubes and somites. In lithium-treated embryos, the segmental expression of AmphiRACK1 in somites became blurry and decreased. Its expression in cerebral vesicles and neural tubes was also weaker or disappeared. In the adult animal, AmphiRACK1 transcripts were detected in the epithelium of midgut diverticulus and gut, wheel organ, gill blood vessels and testis.Vertebrate RACK1 plays a key role in embryonic development. This paper described the cloning, phy- logenetic analysis and developmental expression of AmphiRACK1, the RACK1 homologous gene in amphioxus. Phylogenetic analysis indicated that amphioxus RACK1 was located at the base of verte- brate clade. AmphiRACK1 expression in lithium-treated embryos was also examined. During embryonic development, AmphiRACK1 was expressed strongly in cerebral vesicles, neural tubes and somites. In lithium-treated embryos, the segmental expression of AmphiRACK1 in somites became blurry and decreased. Its expression in cerebral vesicles and neural tubes was also weaker or disappeared. In the adult animal, AmphiRACK1 transcripts were detected in the epithelium of midgut diverticulus and gut, wheel organ, gill blood vessels and testis.

关 键 词：Developmental expression of amphioxus RACK1 FIGURE 

分 类 号：Q953[生物学—动物学]