Electrostatic interaction mechanism on the separation of phenols by non-aqueous capillary electrophoresis  

作　　者：WEI WeiLi YIN YongGuang XIA ZhiNing CHEN ZhiTao LIU WeiQi 

机构地区：[1]Department of Pharmaceutics,College of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400044,China [2]Research Center for Eco-Environmental Science,Chinese Academy of Sciences,Beijing 100081,China [3]Key Laboratory for Biomechanics&Tissue Engineering of Ministry of Education,Chongqing University,Chongqing 400044,China

出　　处：《Science China Chemistry》2007年第1期47-53,共7页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China(Grant No.20375051);the Excellent Young Teachers Program of Ministry of Education(Grant No.[2002]123)

摘　　要：The electrostatic interaction between additive and analyte is of great importance to non-aqueous cap- illary electrophoresis(NACE)separation.Three tetraalkylammonium bromides and acetonitrile were applied as additives and running solvent respectively.The effect of alkyl chain length and concentra- tion of additive on electrostatic interactions was investigated by the separation of phenols.The sepa- ration ability was found to increase with decreasing alkyl chain length of the additive,and the resolu- tion values were increased with increasing additive concentration.The separation was seriously dete- riorated after a little amount of water was added in the running solution.Furthermore,the electrostatic interaction is strong under the conditions of low electron cloud density,weak steric hindrance and multi-interaction sites.Thus,the separation result can be predicted by theoretical analysis,which is helpful for the separation of other substances in NACE based on electrostatic interaction.The electrostatic interaction between additive and analyte is of great importance to non-aqueous capillary electrophoresis (NACE) separation. Three tetraalkylammonium bromides and acetonitrile were applied as additives and running solvent respectively. The effect of alkyl chain length and concentration of additive on electrostatic interactions was investigated by the separation of phenols. The separation ability was found to increase with decreasing alkyl chain length of the additive, and the resolution values were increased with increasing additive concentration. The separation was seriously deteriorated after a little amount of water was added in the running solution. Furthermore, the electrostatic interaction is strong under the conditions of low electron cloud density, weak steric hindrance and multi-interaction sites. Thus, the separation result can be predicted by theoretical analysis, which is helpful for the separation of other substances in NACE based on electrostatic interaction.

关 键 词：NON-AQUEOUS CAPILLARY ELECTROPHORESIS ELECTROSTATIC interaction tetraalkylammoniums PHENOL compounds 

分 类 号：O658.9[理学—分析化学]