The influence of dihydropyridines calcium antagonists on 5-HT-induced intracellular calcium signal  

作　　者：WANG ShuMin GUAN HuaShi FANG Yi MA Ping SUN JianZi LIU LiHong 

机构地区：[1]Marine Drug and Food Institute,Ocean University of China,Qingdao 266003,China [2]Beijing Tongren Hospital,Capital University of Medical Sciences,Beijing 100730,China [3]Chinese PLA General Hospital,Beijing 100853,China [4]Chinese PLA General Hospital of Rocket Forces,Beijing 100088,China

出　　处：《Science China Chemistry》2007年第4期562-567,共6页中国科学（化学英文版）

摘　　要：Confocal laser scanning microscopy (CLSM) was applied to detect the intracellular [Ca2+] variety of fluorescent intension, with Fluo-3/AM fluorescence loaded in SFSMC. The results show that 10 μmol/L Lacidipine can reduce the frequence which 10 μmol/L 5-HT induced [Ca2+] spark in SFSMC of calcium over loading to 50%, and amplitude to 50% or so. We can draw a conclusion that dihydropyridines cal-cium antagonists lacidipine can antagonize the release of intracellular [Ca2+] which 5-HT-induced in dose dependent manner.Confocal laser scanning microscopy (CLSM) was applied to detect the intracellular [Ca2+] variety of fluorescent intension, with Fluo-3/AM fluorescence loaded in SFSMC. The results show that 10 μmol/L Lacidipine can reduce the frequence which 10 μmol/L 5-HT induced [Ca2+] spark in SFSMC of calcium over loading to 50%, and amplitude to 50% or so. We can draw a conclusion that dihydropyridines calcium antagonists lacidipine can antagonize the release of intracellular [Ca2+] which 5-HT-induced in dose dependent manner.

关 键 词：LACIDIPINE calcium SPARKS STOMACH FUNDUS smooth muscle cells confocal laser scanning microscopy 

分 类 号：O621.2[理学—有机化学]