多功能臂大环多胺La(Ⅲ)配合物的合成、表征及催化磷酸二酯BNPP和质粒DNA水解的研究  

Macrocyclic Polyamine La(Ⅲ)Complexes with Multifunctional Arms:Syntheses,Characteristics,Catalytic Hydrolysis for Phosphate Diester and Plasmid DNA

作　　者：向清祥[1,2] 王钦[1] 张愉 陈善勇[1] 余孝其[1] 

机构地区：[1]四川大学化学学院绿色化学与技术教育部重点实验室,四川成都610064 [2]乐山师范学院化学与生命科学系,四川乐山614004

出　　处：《中国科技论文》2006年第1期40-46,共7页China Sciencepaper

基　　金：国家自然科学基金(20372051;20471038和20572075);教育部新世纪优秀人才资助计划;教育部博士点专项基金;四川省杰出青年基金;四川大学学生创新基金

摘　　要：合成了新型的多功能臂大环多胺 La(Ⅲ)配合物。通过分光光度滴定法,研究了大环多胺 La(Ⅲ)配合物催化磷酸二酯 BNPP 的水解反应,结果表明,大环多胺配合物可以有效地催化磷酸二酯 BNPP 水解,水解速率提高5.94×10~4倍,动力学研究还揭示了配体结构对催化水解反应的影响。通过琼脂耱凝胶电泳技术,考察了大环多胺 La(Ⅲ)配合物对质粒 DNA 的催化水解作用,结果表明,大环多胺金属配合物可以有效地促进质粒 DNA 在生理条件下的水解裂解,并讨论了配合物结构对水解反应的影响和水解反应机理。Novel multifunctional arm macrocyclic polyamine La(Ⅲ)complexes have been synthesized.Their catalytic hydrolysis for phosphate diester[bis(p-nitrophenyl)-phosphate](BNPP)have been investigated by UV-visible spectrophotometric titration.The results showed that these La(Ⅲ)complexes could effectively catalyze the hydrolysis of BNPP and the hydrolysis rate enhancement is up to 5.94x104 fold.The kinetic study also implied that the structures of complexes were important influencing factors for the catalytic hydrolysis.The hydrolysis of plasmid DNA(PUC18)by macrocyclic polyamine La(Ⅲ)complexes also has been investigated by the agarose gel electrophoresis.The results indicated that these La(Ⅲ)complexes could effectively promote the cleavage of plasmid DNA(PUC18)at physiological pH and temperature.The structure-activity relationships and the proposed mechanism for the hydrolysis of plasmid DNA(PUC 18)catalyzed by the macrocyclic polyamine La(Ⅲ)complexes was discussed.

关 键 词：大环多胺 配合物 BNPP 水解 DNA 水解裂解 

分 类 号：O641.4[理学—物理化学]