瘢痕成纤维细胞中TGF-β受体与整合素相互作用的实验研究  

The experimental study of mechanism of integrin and TGF-β receptor in hypertrophic scar fibroblasts

作　　者：范志宏[1] 何征宇[1] 马进[1] 侯晓宾[1] 

机构地区：[1]上海第二医科大学附属仁济医院整形外科,上海200127

出　　处：《组织工程与重建外科杂志》2005年第2期79-82,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(30000176)

摘　　要：目的:了解TGF-β受体和整合素在瘢痕增生和挛缩过程中的作用。方法:通过荧光定量PCR法(FQ-PCR)测定经TGF-β受体、整合素和粘着斑激酶(FAK)抗体阻断后培养的瘢痕成纤维细胞TGF-β受体以及整合素表达量的变化。结果:经不同抗体阻断后培养的瘢痕成纤维细胞其TGF-βRI和整合素β1的基因拷贝数均较阴性对照组有不同程度的下降(P<0.05)。结论:TGF-β受体和整合素介导的信号传导途径间可能存在着正反馈的效应,共同促进瘢痕的增生和挛缩。FAK是两条信号传导途径的交汇点和作用的中心环节。Objective: To study the mechanism of TGF- β receptor (TGF- β R) and integrin inhypertrophic scar fibroblasts. Methods: Use fluorescent quantitative PCR assay (FQ-PCR) to com-pare mRNA expression level of TGF- β R and integrins in hypertrophic scar fibroblasts after vari-ous antibody blocking. Results: After various antibody blocking, the mRNA of TGF- β R andintegrins in hypertrophic scarfibroblasts expressed obviously lower than that of control groups (P<0.05). Conclusion: The posi-tive feedback effect may exist between the signal transmit pathway mediated by integrins and TGF-β R. FAK is the center of two signal transmission pathways and the key point of their effect in theproliferation and contracture of hypertrophic scar.

关 键 词：增生性瘢痕 转化生长因子Β受体 整合素 荧光定量PCR 

分 类 号：R622[医药卫生—整形外科]