聚内酯类生物降解性高分子微球可注射细胞支架  

Polylactone-type Polymeric Microspheres Injectable Cell Scaffold

作　　者：王身国 崔文瑾 贝建中 

机构地区：[1]中科院化学所高分子物理与化学国家重点实验室,北京100080

出　　处：《组织工程与重建外科杂志》2005年第1期12-17,共6页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国际863项目(2002AA205021);国家自然科学基金委项目(30370389);科技部高技术发展中心项目(CI-2003-08)

摘　　要：目的:用于组织工程化软骨、骨、脂肪等修复重建和整形用的可注射细胞支架。方法:以聚丙交酯(PLLA)、聚丙交酯/聚乙二醇共聚物(PLE),以及聚己内酯/聚乙二醇/聚丙交酯共聚物(PCEL)等聚内酯类生物降解高分子为基材,采用乳液-溶剂挥发法制成微米级大小、具有一定形态结构的微球。结果:聚内酯材料的组成对微球的形态结构有重要影响,微球的孔结构和稳定剂种类对微球的细胞亲和性有重要影响;含聚乙二醇成分的聚内酯材料可以形成呈多孔结构的微球,且以明胶为稳定剂制备的微球细胞亲和性更好。结论:聚内酯类生物降解高分子微球具有可注射性和良好的细胞亲和性,是一类具有应用前景的可注射细胞支架。Objective: To study a new type of injectable cell scaffold for used in repairing and regen- erating of bone, cartilage and fat in tissue engineering. Methods: By means of emulsion-solvent evaporation technique, microspheres with different morphology which were composed of poly(l- lactide) (PLLA), polylactide-co-poly(ethylene oxide) (PLE) and polycaprolactone–co-poly (ethylene oxide) -co-polylactide (PCEL) respectively were prepared. Results: It was found that composition of the polylactone-type polymer has major effect on morphology of the microspheres. Morphology structures of the microspheres and stabilizer used have important effects on cell affinity of the microspheres. The polymers composed of poly(ethylene oxide) component could form porous struc- tured microspheres. On the other hand the microspheres fabricated in the presence of gelatin as stabilizer showed the better cell affinity. Conclusion: Biodegradable polylactone-type microspheres as injectable cell scaffold have good cell affinity and have potential application in tissue engineering.

关 键 词：聚内酯微球 可注射细胞支架 生物降解性 细胞亲和性 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]