转化生长因子及其受体在体外培养软骨细胞中表达的改变  

Expressional Changes of Transforming Growth Factor and its Receptors in Porcine Chondrocytes Cultured in vitro

作　　者：陈瑾君 刘天一 殷德民 崔磊 刘伟 曹谊林 

机构地区：[1]上海第二把手医科大学组织工程研究中心,上海200011

出　　处：《组织工程与重建外科杂志》2005年第1期22-25,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家973项目04子项目(G1999054304)上海市教委"重中之重"重点学科

摘　　要：目的:探讨软骨细胞中转化生长因子β(transforminggrowthfactor,TGF-β)及其受体TGF-βreceptors(TβRs)的表达在体外传代培养过程中的变化趋势,以及它对软骨细胞特有表型的影响。方法:采用猪耳软骨细胞在体外进行扩增传代,并收集第1～9代的细胞样品,作RT-PCR和Western杂交检测,从mRNA和蛋白水平来反映TGF-β1和TβRs的表达。另取软骨细胞作爬片培养,用免疫组织化学法检测TGF-β1表达。结果:TGF-β1、TβRI和TβRII在第1～3代软骨细胞中表达减少的趋势较为平缓;第5～7代时,有大幅的向下跃迁;在第9代时仅微量表达或消失。与此同时,体外爬片培养软骨细胞仅在原代至第2代可见TGF-β1表达,第3代以后则未见表达。结论:TGF-β1和TβRs表达的减弱与软骨细胞在体外培养过程中的老化进展可能有密切关系。Objective: To determine the relationship between changes in TGFβand TGF-βrecep- tor expression and cell viability in the in vitro cultured chondrocytes of different passages. Methods: Porcine Auricle Chondrocytes were cultured in vitro and harvested from passage 1~9 for RT-PCR and Western blotting analysis of the expression of TGF-β1 and TβR Ⅰ and TβR Ⅱ. TGF-β1 expression in chondrocyte was also examined with immunocytochemistry in passages 1～6 chondrocytes. Results: Expression of TGFβ1 and Tβreceptors in chondrocytes decreased slowly from passage 1 to passage 3, but decreased dramatically during passage 5 and 7 and almost disappeared in passage 9. Immunohistochemstry detected TGFβ1 expression only in passage 0～2 chondrocytes. Conclusion: Decreased expression of TGFβ1 and Tβreceptors may play a key role in the senescence of chondrocytes during their long term culture in vitro.

关 键 词：转化生长因子Β 受体 软骨细胞 

分 类 号：R68[医药卫生—骨科学]