Initiation of primary cell culture from amphioxus Branchiostoma belcheri tsingtauense  

作　　者：王昌留 张士璀 宿烽 王磊 李红岩 

机构地区：[1]School of Life Science, Ludong University [2]Department of Marine Biology, Ocean University of China [3]College of Chemical Engineering, Qingdao University of Science and Technology

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第1期69-73,共5页中国海洋湖沼学报（英文版）

基　　金：Supported by Doctoral Initial Fund of Ludong University (No.43304)

摘　　要：Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25℃.Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C.

关 键 词：cephalochordate in vitro culture tail epithelial cell optimal culture condition 

分 类 号：S965[农业科学—水产养殖]