粒细胞集落刺激因子(G-CSF)对老年急性心肌梗死患者外周血干细胞动员效率的临床观察  

The effects of autologous peripheral blood stem cell mobilization by-CSF in old patients with acute myocardial infarction (AMI)

作　　者：张明[1] 李占全[1] 金元哲 袁龙[1] 张薇薇[1] 崔丽杰[1] 赵红岩[1] 

机构地区：[1]辽宁省人民医院辽宁省心血管病医院心内科心血管病研究室,110016

出　　处：《介入放射学杂志》2004年第S2期93-95,共3页Journal of Interventional Radiology

摘　　要：目的　观察粒细胞集落刺激因子 (G CSF)对老年 (≥ 70岁 )急性心肌梗死 (AMI)患者 ,外周血干细胞动员效率。方法　 10例老年AMI患者予以G CSF 30 0 μg～ 6 0 0 μg d皮下注射 ,连续 5d ,第 6天经美国Baxter公司生产的CS30 0 0PLUS血细胞分离机 ,分离PBSC ,动员前及动员后第 3～ 7天行外周血WBC计数检查 ,经流式细胞仪测定CD34+ 的细胞数量。结果　在动员前及动员后第 3～ 7天外周血中WBC量分别为 6 .75× 10 9 L、2 8.16× 10 9 L、34.93× 10 9 L、34.4 0× 10 9 L、38.93× 10 9 L、2 1.85× 10 9 L ;CD34+ 量分别为 6 .2 5× 10 6 、5 1.10× 10 6 、92 .6 0× 10 6 、10 9.6 5× 10 6 、134.6 9× 10 6 、4 5 .0 9× 10 6 ;在动员前及动员后第 3～ 7天外周血中WBC、CD34+ 量与动员时间变化曲线均显示曲线高峰在动员后第 6天 ;患者外周血中CD34+ 细胞数量与WBC数量变化呈正相关 (r=0 .94 0 ) .结论　老年AMI患者行G CSF动员安全可行 ,外周血中CD34+ 细胞数量与WBC变化曲线高峰均出现在第 6天 ,且WBC与CD34+Objective We Observed the mobilization effects of autologous circulating blood stem cell by G-CSF in old patients(≥70 years old) with acute myocardial infarction (AMI).Methods 10 old patients with AMI were allocated to receive either inclusive type Granulocyte Colony-Stimulating Factor (G-CSF), or excrete type G-CSF to mobilize the stem cell, with either 300μg/day or 600μg/day. The patients received G-CSF by hypodermic injection, and the duration of applying G-CSF was 5 days. In the process of the mobilization of the circulating blood stem cell, the white blood cell (WBC) and CD34 + cell count in the circulating blood should be observed. Results Prior to applying G-CSF and the 3rd、4th、5th、6th、7th after applying G-CSF, the counts of WBC were 6.75×10 9/L、28.16×10 9/L、34.93×10 9/L、34.40×10 9/L、38.93×10 9/L、21.85×10 9/L; the counts of CD34+ cell were 6.25×10 6、51.10×10 6、92.60×10 6、109.65×10 6、134.69×10 6、45.09×10 6 The peak of curve that WBC and CD34 + cell count changed with applying days was at the 6th .The count of CD34 + cell in the circulating blood was positive referent with the count of WBC in the circulating blood(r=0.940)Conclusion In old patients with AMI, the mobilized peak of WBC and CD34 + cell counts changed with applying days was at the 6th, and the count of CD34 + cell in the circulating blood was positive referent with the count of WBC in the circulating blood.

关 键 词：G-CSF 干细胞 CD34+细胞 动员 

分 类 号：R542.22[医药卫生—心血管疾病]