利用RT-PCR法对木槿病叶初步检测  

Initial Detection of Hibiscus Illed Leaves by RT-PCR Method

作　　者：董志[1] 张飞云[1] 张海龙[1] 

机构地区：[1]首都师范大学生命科学学院,北京100037

出　　处：《首都师范大学学报（自然科学版）》2004年第S1期79-82,共4页Journal of Capital Normal University：Natural Science Edition

基　　金：北京市教委科技发展计划面上项目(KW2 0 0 3 10 0 2 810 2 )

摘　　要：本试验利用不同方法对木槿病叶进行总RNA提取效果比较 ,同时用RT PCR方法 ,用广谱性植物病毒TMV、CMV、PXV和PVY的外被蛋白基因设计的引物对染病木槿的花叶病和皱缩病检测 .结果显示 :磁珠式固态样本总RNA快速分离纯化的方法提取的RNA比较完整 ,而Sangon(上海生工 )Trizol法提取总RNA和BioDev(博大泰克 )高效Trizol试剂提取总RNA相比较较差 ;不同模板浓度对PCR反应产物的影响较大 ;探讨了四种病毒对木槿的感染情况 ,分析得知 ,木槿的花叶病和皱缩病可能不是由于其中的病毒引起的 .本文虽然没有证明了木槿是由何种病毒引起的 ,但是对木本植物病毒的检测工作翻开了新的一页 ,并且对我国更多的木本植物的病毒检测将有更重要的意义 .The total RNA of the symptom leaves of Hibiscus were extracted by different methods.The result is clear.And we use coat protein gene of TMV、CMV、PXV and PVY which is broad-spectrum plant virus to detect Hibiscus symptom leaves by RT-PCR method.The result displayed that the method of magnetic bead solid state total RNA extracting is very good,both of Sangon Trizol and BioDev Trizol methods are bad; different concentrations of template have more effects on the production of PCR.From the above analysis,we know that leaves symptom of hibiscus were not caused by four virus tested.Though we have not proved which virus led to infection of hibiscus,while advances in methods of detection of woody plants have been discussed,besides which play an important significance for detecting more virus of woody plants.

关 键 词：木槿 RT-PCR 植物病毒 检测 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]