利用RAPD分子标记对苦竹与近缘竹亲缘关系的研究  

A study on genetic relationship in the bitter bamboo and its relatives by using random amplified polymorphic DNA (RAPD) markers

作　　者：杨萍[1] 方伟[1,2] 林新春[1,2] 

机构地区：[1]浙江林学院现代森林培育技术重点实验室 [2]浙江林学院竹类研究所,浙江临安311300

出　　处：《云南大学学报（自然科学版）》2004年第S2期63-67,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：浙江省科委资助项目(苦竹等多用途经济竹类深度开发研究)

摘　　要：采用RAPD分子标记研究了苦竹及近缘竹(17个竹种)的亲缘关系.筛选出20个最佳引物,共计扩增304个片段,计算遗传相似系数,建立UPGMA聚类图,结果表明:大明竹(Pleioblastusgramineus(Beam)Nakai)与小黄苦竹(OligostachyumfujinensisC.P.WangetG.H.Ye)的遗传距离最大为0.6356,衢县苦竹(P.juxianensisWenetal)与硬头苦竹(P.longifimbnatusS.Y.Chen)的遗传距离最小为0.2490.The relationship among 17 species in the Bitter bamboo and its relatives was studied by means of Random Amplified Polymorphic DNA (RAPD) Markers. Three hundred four polymorphic loic were amplified with 20 primers, by popgen 32 program, Nei's gentic similarity coefficients were generated and dendrogram was constructed using UPGMA. The result shows that: The longest Nei(1978)genetic distance was 0. 635 6 between pleioblastus gramineus (Beam) Nakai and Oligostachyum fujinensis C. P. Wang et G. H. Ye; The shortest Nei(1978)genetic distance was 0.635 6 between P. juxianensis Wen et al and P.langifimb-natus S. Y. Chen.

关 键 词：苦竹与近缘竹 RAPD 亲缘关系 

分 类 号：S795.9[农业科学—林木遗传育种]